PMID- 8051160
OWN - NLM
STAT- MEDLINE
DCOM- 19940906
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 269
IP  - 32
DP  - 1994 Aug 12
TI  - Androgen and glucocorticoid receptors interact with insulin degrading enzyme.
PG  - 20622-8
AB  - We recently identified a protein, designated receptor accessory factor (RAF), 
      that interacts directly with and enhances the DNA binding of androgen (AR) and 
      glucocorticoid (GR) receptor peptide fragments. To determine its identity, RAF 
      was purified from HeLa cell extracts by anion-exchange and DNA affinity 
      chromatography. RAF activity co-purified with a 110-kDa protein, the partial 
      amino acid sequence of which shares 97.5% identity with insulin degrading enzyme 
      (IDE), a metalloendoprotease implicated in the intracellular degradation of 
      insulin. The identity of RAF was confirmed in gel shift assays by demonstrating 
      that AR.RAF and GR.RAF DNA complexes shifted to a slower mobility in the presence 
      of an IDE antibody. Purified preparations of RAF had insulin degrading activity, 
      and this activity was inhibited by AR. In addition, the interaction of AR or GR 
      with RAF was competed by insulin and bacitracin, a competitive inhibitor of IDE. 
      The interactions of AR and GR with IDE may have important implications for both 
      insulin- and steroid-mediated signaling.
FAU - Kupfer, S R
AU  - Kupfer SR
AD  - Laboratories for Reproductive Biology, University of North Carolina School of 
      Medicine, Chapel Hill 27599.
FAU - Wilson, E M
AU  - Wilson EM
FAU - French, F S
AU  - French FS
LA  - eng
GR  - HD04466/HD/NICHD NIH HHS/United States
GR  - HD16910/HD/NICHD NIH HHS/United States
GR  - K08-HD00963/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Insulin)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (nuclear accessory protein, monkey)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.55 (pitrilysin)
SB  - IM
MH  - Base Sequence
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Endopeptidases/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Insulin/metabolism
MH  - *Metalloendopeptidases
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/isolation & purification/metabolism
MH  - Oligodeoxyribonucleotides
MH  - Receptors, Androgen/*metabolism
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Signal Transduction
EDAT- 1994/08/12 00:00
MHDA- 1994/08/12 00:01
CRDT- 1994/08/12 00:00
PHST- 1994/08/12 00:00 [pubmed]
PHST- 1994/08/12 00:01 [medline]
PHST- 1994/08/12 00:00 [entrez]
AID - S0021-9258(17)32038-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1994 Aug 12;269(32):20622-8.