PMID- 8051615
OWN - NLM
STAT- MEDLINE
DCOM- 19940906
LR  - 20190710
IS  - 0022-3573 (Print)
IS  - 0022-3573 (Linking)
VI  - 46
IP  - 4
DP  - 1994 Apr
TI  - A novel 11 beta-hydroxysteroid dehydrogenase inhibitor contained in saiboku-to, a 
      herbal remedy for steroid-dependent bronchial asthma.
PG  - 305-9
AB  - To identify the inhibitor of prednisolone metabolism contained in Saiboku-To, we 
      conducted in-vitro experiments of 11 beta-hydroxysteroid dehydrogenase (11 
      beta-HSD), using rat liver homogenate and cortisol as a typical substrate. We 
      studied the effects of ten herbal constituents on 11 beta-HSD. Five herbal 
      extracts showed inhibitory activity with Glycyrrhiza glabra > Perillae frutescens 
      > Zizyphus vulgaris > Magnolia officinalis > Scutellaria baicalensis. This 
      suggests that unknown 11 beta-HSD inhibitors are contained in four herbs other 
      than G. glabra which contains a known inhibitor, glycyrrhizin (and glycyrrhetinic 
      acid). Seven chemical constituents which have been identified as the major 
      urinary products of Saiboku-To in healthy and asthmatic subjects were studied; 
      magnolol derived from M. officinalis showed the most potent inhibition of the 
      enzyme (IC50, 1.8 x 10(-4) M). Although this activity was less than that of 
      glycyrrhizin, the inhibition mechanism (non-competitive) was different from a 
      known competitive mechanism. These results suggest that magnolol might contribute 
      to the inhibitory effects of Saiboku-To on prednisolone metabolism through 
      inhibition of 11 beta-HSD.
FAU - Homma, M
AU  - Homma M
AD  - Department of Clinical Pharmacology, Tokyo College of Pharmacy, Japan.
FAU - Oka, K
AU  - Oka K
FAU - Niitsuma, T
AU  - Niitsuma T
FAU - Itoh, H
AU  - Itoh H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glucocorticoids)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lignans)
RN  - 0 (saiboku-to)
RN  - 001E35HGVF (magnolol)
RN  - 11513CCO0N (honokiol)
RN  - 6FO62043WK (Glycyrrhizic Acid)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - P540XA09DR (Glycyrrhetinic Acid)
RN  - V27W9254FZ (Cortisone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/isolation & 
      purification/pharmacology/therapeutic use
MH  - Asthma/*drug therapy
MH  - Biphenyl Compounds/isolation & purification/pharmacology/therapeutic use
MH  - Chromatography, High Pressure Liquid
MH  - Cortisone/biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology/therapeutic use
MH  - Glucocorticoids/analysis
MH  - Glycyrrhetinic Acid/analogs & derivatives/pharmacology
MH  - Glycyrrhizic Acid
MH  - Hydrocortisone/metabolism
MH  - Hydroxysteroid Dehydrogenases/*antagonists & inhibitors
MH  - Immunosuppressive Agents/chemistry/*pharmacology/therapeutic use
MH  - *Lignans
MH  - Liver/*drug effects/enzymology
MH  - Male
MH  - *Medicine, Kampo
MH  - Rats
MH  - Rats, Wistar
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1111/j.2042-7158.1994.tb03799.x [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 1994 Apr;46(4):305-9. doi: 10.1111/j.2042-7158.1994.tb03799.x.