PMID- 8051713
OWN - NLM
STAT- MEDLINE
DCOM- 19940906
LR  - 20131121
IS  - 0098-4108 (Print)
IS  - 0098-4108 (Linking)
VI  - 42
IP  - 4
DP  - 1994 Aug
TI  - Immunotoxicity of particulate lead: in vitro exposure alters pulmonary macrophage 
      tumor necrosis factor production and activity.
PG  - 377-92
AB  - Rabbit pulmonary macrophages were exposed in vitro to particulate lead oxide 
      (PbO) for periods of up to 72 h and then assayed for the activity of tumor 
      necrosis factor-alpha (TNF alpha) released after stimulation with 
      lipopolysaccharide (LPS). The levels of TNF alpha obtained from PbO-treated cells 
      were decreased in a dose-dependent manner as compared with metal-free control 
      cells for each time point examined. Cells treated simultaneously with both LPS 
      and PbO yielded less monokine than did cells receiving LPS alone. In addition, 
      incubation of cell-free TNF alpha with PbO resulted in a diminution of 
      cytotoxicity directed against TNF alpha-sensitive tumor target cells. Macrophage 
      burdens of PbO particles increased with both the length of incubation and 
      concentration of PbO used; increases in cellular lead burdens were paralleled by 
      reductions in cell viability. Thus, under in vitro conditions, PbO affects the 
      levels of the immunoregulatory monokine TNF alpha and also disrupts its cytotoxic 
      properties after release from activated macrophages.
FAU - Cohen, M D
AU  - Cohen MD
AD  - Department of Environmental Medicine, New York University Medical Center, Tuxedo 
      10987.
FAU - Yang, Z
AU  - Yang Z
FAU - Zelikoff, J T
AU  - Zelikoff JT
LA  - eng
GR  - NIEHS ES00260/ES/NIEHS NIH HHS/United States
GR  - NIEHS ES04627/ES/NIEHS NIH HHS/United States
GR  - NIEHS ES07065/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Toxicol Environ Health
JT  - Journal of toxicology and environmental health
JID - 7513622
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oxides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 2P299V784P (Lead)
RN  - 4IN6FN8492 (lead oxide)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Lead/pharmacokinetics/*toxicity
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Alveolar/drug effects/*metabolism
MH  - Male
MH  - Oxides/pharmacokinetics/*toxicity
MH  - Rabbits
MH  - Specific Pathogen-Free Organisms
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/drug effects/*metabolism
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1080/15287399409531889 [doi]
PST - ppublish
SO  - J Toxicol Environ Health. 1994 Aug;42(4):377-92. doi: 10.1080/15287399409531889.