PMID- 8052140
OWN - NLM
STAT- MEDLINE
DCOM- 19940908
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 43
IP  - 8
DP  - 1994 Aug
TI  - Lack of association of the transporter associated with antigen processing with 
      Japanese insulin-dependent diabetes mellitus.
PG  - 1013-7
AB  - The transporter associated with antigen processing (TAP) encoded in the major 
      histocompatibility complex (MHC) class II region is a molecule required for 
      endogenous antigen processing. We have typed TAP polymorphism in 95 Japanese 
      patients with insulin-dependent diabetes mellitus (DDM) and 75 normal controls. 
      Amino acid substitutions at positions 333 and 637 of TAP1 and at positions 379, 
      665, and 687 of TAP2 were typed by the polymerase chain reaction 
      (PCR)-sequence-specific oligonucleotide method. In addition, DNA typing of human 
      leukocyte antigen (HLA)-DQA1 and -DQB1 loci was performed by the PCR-restriction 
      fragment length polymorphism method. There was no significant difference between 
      IDDM patients and normal controls in the frequencies of TAP1 and TAP2 alleles. On 
      the contrary, the HLA-DQ locus showed a strong association with IDDM in the same 
      series of subjects. The frequencies of HLA-DQA1*0301 and -DQB1*0401 were 
      increased significantly and those of HLA-DQA1*0103, -DQB1*0501, -DQB1*0601 and 
      -DQB1*0602 were decreased significantly in Japanese IDDM patients compared with 
      normal controls. Positive linkage disequilibrium was observed between 
      HLA-DQB1*0303 and TAP2C and between HLA-DQB1*0401 and TAP2B. Negative linkage 
      disequilibrium was observed between HLA-DQA1*0103 and TAP2A. Even when subjects 
      with HLA-DQA1*0103, -DQA1*0301, -DQB1*0302, -DQB1*0303, and -DQB1*0401 were 
      considered separately, no significant differences was found in the distribution 
      of TAP1 and TAP2 alleles between IDDM patients and normal controls. We conclude 
      that it is not TAP but HLA-DQ that exhibits a primary association with Japanese 
      IDDM.
FAU - Nakanishi, K
AU  - Nakanishi K
AD  - Department of Endocrinology and Metabolism, Toranomon Hospital, Tokyo, Japan.
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Murase, T
AU  - Murase T
FAU - Kosaka, K
AU  - Kosaka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Carrier Proteins)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alleles
MH  - Antigen Presentation
MH  - Carrier Proteins/*genetics
MH  - Child
MH  - Diabetes Mellitus, Type 1/ethnology/*genetics/*immunology
MH  - Female
MH  - Genes, MHC Class II/genetics
MH  - HLA-DQ Antigens/*genetics
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Humans
MH  - Japan
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 0026-0495(94)90182-1 [pii]
AID - 10.1016/0026-0495(94)90182-1 [doi]
PST - ppublish
SO  - Metabolism. 1994 Aug;43(8):1013-7. doi: 10.1016/0026-0495(94)90182-1.