PMID- 8052508
OWN - NLM
STAT- MEDLINE
DCOM- 19940908
LR  - 20190818
IS  - 0031-1820 (Print)
IS  - 0031-1820 (Linking)
VI  - 108 ( Pt 5)
DP  - 1994 Jun
TI  - Characterization and localization of Schistosoma mansoni calreticulin Sm58.
PG  - 527-32
AB  - Recombinant Schistosoma mansoni calreticulin (SmCaR) was expressed in Escherichia 
      coli, using the glutathione S-transferase fusion protein, and its Ca(2+)-binding 
      capacity was determined. Results obtained by a 45Ca2+ overlay technique showed 
      that Ca(2+)-binding site(s) were present in the recombinant CaR indicating that 
      proper folding of the protein was obtained using this system. An antiserum raised 
      against the recombinant SmCaR showed that the native protein (Sm58) was expressed 
      in all stages of the life-cycle from cercariae to the adult worm and in the egg. 
      However, SmCaR seems to be a developmentally regulated protein whose expression 
      can be used to study the post-transformational differentiation of the 
      schistosomulum. Localization of SmCaR demonstrated that the majority of SmCaR was 
      expressed in the epithelia of the digestive duct and in the genital organs. These 
      results suggest that SmCaR, by regulating the Ca2+ concentration, may play an 
      important role during cell proliferation. Finally the presence of SmCaR in 
      miracidia and in the genital organs suggests that the antibody response directed 
      against this protein could interfere in egg production.
FAU - Khalife, J
AU  - Khalife J
AD  - Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut 
      Pasteur, Lille, France.
FAU - Liu, J L
AU  - Liu JL
FAU - Pierce, R
AU  - Pierce R
FAU - Porchet, E
AU  - Porchet E
FAU - Godin, C
AU  - Godin C
FAU - Capron, A
AU  - Capron A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Antigens, Helminth)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calreticulin)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoproteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Helminth
MH  - Biomphalaria
MH  - Calcium-Binding Proteins/genetics/immunology/*metabolism
MH  - Calreticulin
MH  - Cricetinae
MH  - Escherichia coli/genetics
MH  - Female
MH  - Immunohistochemistry
MH  - Liver/metabolism
MH  - Male
MH  - Mesocricetus
MH  - Molecular Sequence Data
MH  - Recombinant Fusion Proteins/genetics/immunology/metabolism
MH  - Ribonucleoproteins/genetics/immunology/*metabolism
MH  - Schistosoma mansoni/genetics/growth & development/*metabolism
MH  - Schistosomiasis mansoni/metabolism
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1017/s0031182000077398 [doi]
PST - ppublish
SO  - Parasitology. 1994 Jun;108 ( Pt 5):527-32. doi: 10.1017/s0031182000077398.