PMID- 8055774
OWN - NLM
STAT- MEDLINE
DCOM- 19940912
LR  - 20190914
IS  - 0378-3782 (Print)
IS  - 0378-3782 (Linking)
VI  - 33
IP  - 2
DP  - 1993 Jun
TI  - Appraisal of fluorescence in situ hybridization (FISH) techniques in prenatal 
      diagnosis.
PG  - 101-8
AB  - The reliability of FISH was appraised using probes for the X and Y chromosome and 
      for chromosomes 12 and 18 in prenatal and adult interphase nuclei. Detection of a 
      single hybridization spot proved to be quite reliable (80-92% positive nuclei). 
      Detection of two hybridization spots was more difficult; percentages of nuclei 
      showing two signals varied between 62-72%. The percentages of nuclei with the 
      correct number of spots was higher in the metaphases occasionally found. Thus, 
      FISH may complement but not replace cytogenetic analysis. For sex determination 
      and for the detection of mosaicism, we suggest the use of two different probes in 
      separate regions of the same preparation.
FAU - Grao, P
AU  - Grao P
AD  - Departament de Biologia Cellular i Fisiologia, Universitat Autonoma de Barcelona, 
      Bellaterra, Spain.
FAU - Guitart, M
AU  - Guitart M
FAU - Coll, M D
AU  - Coll MD
FAU - Egozcue, J
AU  - Egozcue J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Early Hum Dev
JT  - Early human development
JID - 7708381
RN  - 0 (DNA Probes)
SB  - IM
MH  - Amniotic Fluid/cytology
MH  - Chorionic Villi
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 18
MH  - DNA Probes
MH  - Female
MH  - Fetal Blood/cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Sex Chromosomes
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 0378-3782(93)90205-9 [pii]
AID - 10.1016/0378-3782(93)90205-9 [doi]
PST - ppublish
SO  - Early Hum Dev. 1993 Jun;33(2):101-8. doi: 10.1016/0378-3782(93)90205-9.