PMID- 8055906
OWN - NLM
STAT- MEDLINE
DCOM- 19940913
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 223
IP  - 2
DP  - 1994 Jul 15
TI  - A peptide C-terminal to the second Zn finger of human vitamin D receptor is able 
      to specify nuclear localization.
PG  - 381-7
AB  - A peptide of 27 amino acids, VDR(102-76), representing residues 76-102 
      immediately C-terminal to the second Zn finger of human vitamin D receptor (hVDR) 
      was conjugated to fluorescein-labelled IgG using a bifunctional coupling reagent, 
      m-maleimidobenzoyl n-hydroxysuccinimide. Upon microinjection into the cytoplasm 
      of human osteosarcoma MG-63 cells, the chimeras accumulated in the nuclei. This 
      transport was arrested by chilling or energy depletion. Two other peptides, 
      VDR(80-67), spanning the N-terminal part of VDR(102-76), and VDR(108-97), 
      spanning the C-terminal part of VDR(102-76), were not able to target the linked 
      proteins to the nuclei. SV40(135-112), a peptide containing a well-characterized 
      nuclear localization sequence (amino acids 112-135) of simian virus 40 (SV40) 
      large T-antigen, caused complete nuclear accumulation under the same conditions. 
      Wheat germ agglutinin, which inhibits SV40(135-112) transport, also inhibited the 
      nuclear accumulation of VDR(102-76) as did energy depletion.
FAU - Luo, Z
AU  - Luo Z
AD  - Department of Biochemistry & Biotechnology, University of Kuopio, Finland.
FAU - Rouvinen, J
AU  - Rouvinen J
FAU - Maenpaa, P H
AU  - Maenpaa PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Wheat Germ Agglutinins)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Polyomavirus Transforming/chemistry/metabolism
MH  - Biological Transport
MH  - Cell Nucleus/*metabolism
MH  - Cold Temperature
MH  - Cross-Linking Reagents
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Energy Metabolism
MH  - Fluorescein-5-isothiocyanate
MH  - Humans
MH  - Immunoglobulin G
MH  - Molecular Sequence Data
MH  - Osteosarcoma
MH  - Peptide Fragments/chemistry/*metabolism
MH  - Receptors, Calcitriol/chemistry/*metabolism
MH  - Tumor Cells, Cultured
MH  - Wheat Germ Agglutinins/pharmacology
MH  - *Zinc Fingers
EDAT- 1994/07/15 00:00
MHDA- 1994/07/15 00:01
CRDT- 1994/07/15 00:00
PHST- 1994/07/15 00:00 [pubmed]
PHST- 1994/07/15 00:01 [medline]
PHST- 1994/07/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1994.tb19004.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1994 Jul 15;223(2):381-7. doi: 10.1111/j.1432-1033.1994.tb19004.x.