PMID- 8060345
OWN - NLM
STAT- MEDLINE
DCOM- 19940912
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 202
IP  - 3
DP  - 1994 Aug 15
TI  - Tumor necrosis factor-alpha (TNF-alpha) inhibits expression of mouse placental 
      lactogen-II through TNF-alpha type-I but not type-II receptor.
PG  - 1599-605
AB  - The aim of this study was to determine whether TNF-alpha inhibits mPL-II 
      secretion through TNF-RI or TNF-RII, and to investigate the gestational profile 
      of TNF-RI and TNF-RII gene expression. The mouse trophoblast cells from day 12 
      pregnancy were cultured with or without agonistic polyclonal antibodies directed 
      against the individual TNF-alpha receptors, and mPL-II secretion in the medium 
      was assessed by RIA. Anti-TNF-RI antibody significantly inhibited the mPL-II 
      secretion in a dose- and time-dependent manner, but anti-TNF-RII antibody did 
      not. Moreover, the TNF-RII antibody did not influence the inhibitory effect of 
      the TNF-RI antibody on mPL-II secretion. TNF-RI antibody inhibited the mPL-II 
      gene expression by Northern blot analysis. Amount of the gene expression of 
      TNF-RI in the second half of pregnancy was higher than those in the first half of 
      pregnancy both in vitro and in vivo, although the gene expression of TNF-RII was 
      not detectable by Northern blot analysis using poly(A)+RNA. These results suggest 
      that TNF-RI is a main receptor for TNF-alpha in mouse placenta and that TNF-alpha 
      may have an important role in regulating mPL-II secretion after midpregnancy.
FAU - Yamaguchi, M
AU  - Yamaguchi M
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Kurachi, H
AU  - Kurachi H
FAU - Tadokoro, C
AU  - Tadokoro C
FAU - Sakata, M
AU  - Sakata M
FAU - Yoshimoto, Y
AU  - Yoshimoto Y
FAU - Masumoto, N
AU  - Masumoto N
FAU - Tasaka, K
AU  - Tasaka K
FAU - Miyake, A
AU  - Miyake A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9035-54-5 (Placental Lactogen)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation
MH  - Mice
MH  - Placenta/*metabolism
MH  - Placental Lactogen/*genetics/metabolism
MH  - Pregnancy
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/08/15 00:00
MHDA- 1994/08/15 00:01
CRDT- 1994/08/15 00:00
PHST- 1994/08/15 00:00 [pubmed]
PHST- 1994/08/15 00:01 [medline]
PHST- 1994/08/15 00:00 [entrez]
AID - S0006-291X(84)72115-2 [pii]
AID - 10.1006/bbrc.1994.2115 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Aug 15;202(3):1599-605. doi: 
      10.1006/bbrc.1994.2115.