PMID- 8061656
OWN - NLM
STAT- MEDLINE
DCOM- 19940919
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 8
IP  - 4
DP  - 1994 May
TI  - 4-Amino-4-deoxy-L-arabinose in LPS of enterobacterial R-mutants and its possible 
      role for their polymyxin reactivity.
PG  - 329-41
AB  - The content of 4-amino-4-deoxy-L-arabinopyranose (L-Arap4N) and the phosphate 
      substitution pattern of the LPS of various strains from Salmonella minnesota, 
      Yersinia enterocolitica and Proteus mirabilis was determined by GC/MS, HPLC and 
      31P-NMR. These data allowed us to examine the possible role of these components 
      for the polymyxin B-binding capacity of LPS and for the minimal inhibiting 
      concentration (MIC) and the minimal bactericidal concentration (MBC) of 
      polymyxins B and E towards the respective R-mutants. Contrary to other 
      investigated Re-, Rd- and Rc-mutants of S. minnesota, strain R595 (Re-mutant) 
      showed about a 90% substitution of the ester-linked phosphate-group with 
      L-Arap4N, whereas the L-Arap4N content of the other S. minnesota strains amounted 
      to 17-25%. Neither the binding capacity of LPS to polymyxin B, determined by a 
      bioassay, nor the MIC- and MBC-values of the R-mutants were significantly 
      affected by this alteration. Similar results were obtained after using the 
      temperature-dependent changes in the L-Arap4N-content and phosphate substitution 
      pattern of Y. enterocolitica 75R. In order to explore the relevant polymyxin B 
      binding site, lipid A samples with or without substitution of their ester-linked 
      phosphate group were prepared and subjected to the polymyxin-binding assay. The 
      results obtained so far indicated that the inner core bound L-Arap4N, detected in 
      all resistant strains investigated, may play a decisive role in the decreased 
      binding of polymyxin B, responsible for the bacterial resistance towards 
      polymyxin(s).
FAU - Boll, M
AU  - Boll M
AD  - Max-Planck-Institut fur Immunbiologie, Freiburg, FRG.
FAU - Radziejewska-Lebrecht, J
AU  - Radziejewska-Lebrecht J
FAU - Warth, C
AU  - Warth C
FAU - Krajewska-Pietrasik, D
AU  - Krajewska-Pietrasik D
FAU - Mayer, H
AU  - Mayer H
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Amino Sugars)
RN  - 0 (Lipid A)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polymyxins)
RN  - 0 (Sugar Acids)
RN  - 1069-03-0 (2-keto-3-deoxyoctonate)
RN  - 143600-83-3 (3-deoxy-2-octulosonic acid(2)-lipid IV(A))
RN  - 33406-49-4 (4-amino-4-deoxyarabinose)
SB  - IM
MH  - Amino Sugars/*analysis
MH  - Enterobacteriaceae/*chemistry/drug effects/genetics
MH  - Lipid A/analogs & derivatives/chemistry/genetics
MH  - Lipopolysaccharides/*chemistry/metabolism
MH  - Microbial Sensitivity Tests
MH  - Mutation
MH  - Polymyxins/metabolism/*pharmacology
MH  - Sugar Acids/chemistry
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1111/j.1574-695X.1994.tb00460.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 1994 May;8(4):329-41. doi: 
      10.1111/j.1574-695X.1994.tb00460.x.