PMID- 8062220
OWN - NLM
STAT- MEDLINE
DCOM- 19940922
LR  - 20191210
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 83
IP  - 1-2
DP  - 1994 Aug 15
TI  - Cell type specific patterns of mRNA splicing in hepatoma cells transfected with 
      the mutated albumin minigene of Nagase analbuminemic rats.
PG  - 221-7
AB  - We constructed an SV40-derived expression vector containing a mutated albumin 
      minigene of Nagase analbuminemic rats (NAR), and introduced it into cultured 
      cells. Transient expression of the minigene mRNA was determined by RT-PCR. Three 
      kinds of aberrant mRNAs were expressed by the non-hepatic cells COS-1, and the 
      undifferentiated human hepatoma cells HLE, transfected with the minigene. Their 
      predominant mRNA lacked exon H (delta H), while mRNAs lacking exons H and I, or 
      exons G and H were less abundant. This pattern of the mRNAs was similar to that 
      of albumin mRNAs in the liver of old NAR. In contrast, a differentiated type of 
      human hepatoma cell line, HepG2, expressed only delta H mRNA, like young NAR. 
      These findings indicate that the expression system of the mutated minigene in 
      cultured hepatoma cells is useful for understanding two-exon-skipping of albumin 
      pre-mRNA of NAR.
FAU - Sugiyama, K
AU  - Sugiyama K
AD  - Investigative Cancer Treatment Division, National Cancer Center Research 
      Institute, Chiba, Japan.
FAU - Hitomi, Y
AU  - Hitomi Y
FAU - Adachi, H
AU  - Adachi H
FAU - Esumi, H
AU  - Esumi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - DNA Primers/chemistry
MH  - Exons
MH  - Gene Expression
MH  - Humans
MH  - In Vitro Techniques
MH  - Liver Neoplasms, Experimental/*genetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Mutant Strains/*genetics
MH  - Serum Albumin/*genetics
MH  - Transfection
EDAT- 1994/08/15 00:00
MHDA- 1994/08/15 00:01
CRDT- 1994/08/15 00:00
PHST- 1994/08/15 00:00 [pubmed]
PHST- 1994/08/15 00:01 [medline]
PHST- 1994/08/15 00:00 [entrez]
AID - 0304-3835(94)90323-9 [pii]
AID - 10.1016/0304-3835(94)90323-9 [doi]
PST - ppublish
SO  - Cancer Lett. 1994 Aug 15;83(1-2):221-7. doi: 10.1016/0304-3835(94)90323-9.