PMID- 8062894
OWN - NLM
STAT- MEDLINE
DCOM- 19940919
LR  - 20190909
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 53
IP  - 1
DP  - 1994 Jul
TI  - Peripheral blood mononuclear cells from patients with rheumatoid arthritis 
      suppress erythropoiesis in vitro via the production of tumor necrosis factor 
      alpha.
PG  - 26-30
AB  - Anemia is a frequent manifestation of rheumatoid arthritis, with a probably 
      multifactorial etiology. We investigated the effect of peripheral blood 
      mononuclear cell supernatants (PBMCS) from rheumatoid arthritis (RA) patients on 
      hematopoietic colony formation in vitro, by using a methylcellulose assay. PBMCS 
      from patients suppress in vitro erythroid (BFU-E), mixed-lineage (CFU-GEMM) and 
      to a lesser degree granulocyte-macrophage (CFU-GM) progenitors. PBMCS from anemic 
      RA patients were more suppressive for BFU-E than those from non-anemic patients. 
      Addition of antibodies to tumor-necrosis factor alpha (TNF alpha) almost 
      completely reversed the inhibition of BFU-E and CFU-GEMM, but had little effect 
      on the CFU-GM colony formation. Antibodies to interferon-gamma (IFN gamma) and 
      interleukin-1 (IL-1) were not effective. The above data suggest that PBMCS from 
      RA patients suppress in vitro erythropoiesis via the production of TNF alpha; a 
      pathogenetic role for TNF alpha in the anemia of RA can be implied.
FAU - Katevas, P
AU  - Katevas P
AD  - Department of Internal Medicine, Patras University Medical School, Greece.
FAU - Andonopoulos, A P
AU  - Andonopoulos AP
FAU - Kourakli-Symeonidis, A
AU  - Kourakli-Symeonidis A
FAU - Manopoulou, E
AU  - Manopoulou E
FAU - Lafi, T
AU  - Lafi T
FAU - Makri, M
AU  - Makri M
FAU - Zoumbos, N C
AU  - Zoumbos NC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Antibodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies/pharmacology
MH  - Arthritis, Rheumatoid/*blood
MH  - Erythroid Precursor Cells/pathology
MH  - *Erythropoiesis
MH  - Granulocytes/pathology
MH  - Hematopoietic Stem Cells/pathology
MH  - Humans
MH  - Leukocytes, Mononuclear/*physiology
MH  - Macrophages/pathology
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/*biosynthesis
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1111/j.1600-0609.1994.tb00175.x [doi]
PST - ppublish
SO  - Eur J Haematol. 1994 Jul;53(1):26-30. doi: 10.1111/j.1600-0609.1994.tb00175.x.