PMID- 8063420
OWN - NLM
STAT- MEDLINE
DCOM- 19940921
LR  - 20220321
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 62
IP  - 9
DP  - 1994 Sep
TI  - Localized expression of mRNA for phagocyte-specific chemotactic cytokines in 
      human periodontal infections.
PG  - 4005-14
AB  - In bacterial infections, mononuclear and polymorphonuclear phagocytes are key 
      components of host defenses. Recent investigations have indicated that chemokines 
      are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) 
      attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant 
      protein-1 (MCP-1) is selective for cells of the monocyte/macrophage lineage. In 
      this investigation, we analyzed the in situ expression of IL-8 and MCP-1 mRNAs in 
      human periodontal infections. Specific mRNA was detected by in situ hybridization 
      using 35S-labeled riboprobes in frozen tissue sections. Phagocytes (PMNs and 
      macrophages) were specifically detected as elastase-positive or CD68+ cells by a 
      three-stage immunoperoxidase technique. Results indicated that expression of 
      phagocyte-specific cytokines was confined to selected tissue locations and, in 
      general, paralleled phagocyte infiltration. In particular, IL-8 expression was 
      maximal in the junctional epithelium adjacent to the infecting microorganisms; 
      PMN infiltration was more prominent in the same area. MCP-1 was expressed in the 
      chronic inflammatory infiltrate and along the basal layer of the oral epithelium. 
      Cells of the monocyte/macrophage lineage were demonstrated to be present in the 
      same areas. The observed expression pattern may be the most economic way to 
      establish a cell-type-selective chemotactic gradient within the tissue that is 
      able to effectively direct polymorphonuclear phagocyte migration toward the 
      infecting microorganisms and modulate mononuclear phagocyte infiltration in the 
      surrounding tissues. This process may optimize host defenses and contribute to 
      containing leukocyte infiltration to the infected and inflamed area, thus 
      limiting tissue damage.
FAU - Tonetti, M S
AU  - Tonetti MS
AD  - Department of Peridontology and Fixed Prosthodontics, School of Dental Medicine, 
      University of Bern, Switzerland.
FAU - Imboden, M A
AU  - Imboden MA
FAU - Gerber, L
AU  - Gerber L
FAU - Lang, N P
AU  - Lang NP
FAU - Laissue, J
AU  - Laissue J
FAU - Mueller, C
AU  - Mueller C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*genetics
MH  - Gingivitis/*metabolism/pathology
MH  - Humans
MH  - Interleukin-8/*genetics
MH  - Neutrophils/physiology
MH  - Periodontitis/*metabolism
MH  - Phagocytes/*physiology
MH  - RNA, Messenger/*analysis
PMC - PMC303060
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
PMCR- 1994/09/01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PHST- 1994/09/01 00:00 [pmc-release]
AID - 10.1128/iai.62.9.4005-4014.1994 [doi]
PST - ppublish
SO  - Infect Immun. 1994 Sep;62(9):4005-14. doi: 10.1128/iai.62.9.4005-4014.1994.