PMID- 8067466
OWN - NLM
STAT- MEDLINE
DCOM- 19940921
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 2 Pt 2
DP  - 1994 Aug
TI  - Control of red cell function of late chick embryos: role of extracellular ATP/AMP 
      and egg size.
PG  - R542-8
AB  - Hypoxia is the alleged stimulus for initiation of increase of carbonic anhydrase 
      II (CAII) and 2,3-diphosphoglycerate (2,3-DPG) synthesis of red blood cells from 
      late chick embryos. The PO2-dependent regulation of red cell metabolism is 
      mediated by unknown humoral factors [Million et al., Am. J. Physiol. 261 
      (Regulatory Integrative Comp. Physiol. 30): R1188-R1196, 1991]. In the present 
      investigation we have analyzed whether interindividual differences in egg size 
      (which result in different surface area-to-mass ratios) affect the timing of 
      initiation of 2,3-DPG and CAII synthesis in late chick embryos. We also 
      investigated the effect of extracellular adenine nucleotides on red cell organic 
      phosphate pattern and O2 affinity to test whether the inhibitory effect of normal 
      or elevated PO2 on 2,3-DPG synthesis and the concomitant increase of ATP (and O2 
      half-saturation pressure) can be mimicked by these agents. The results show that 
      differences in egg size affect the timing of CAII and 2,3-DPG synthesis, 
      indicating that PO2-dependent regulation of red cell function allows adjustment 
      to the properties of the individual egg. We also found that extracellular ATP, 
      which is rapidly degraded to AMP by red cell ectoenzymes, can alter the red cell 
      phosphate pattern and O2 affinity, i.e., significantly increase red cell ATP, 
      decrease red cell 2,3-DPG and O2 affinity, and thus mimic the effect of normoxia 
      and hyperoxia. These findings suggest that extracellular adenine nucleotides may 
      be involved in the PO2-dependent regulation of embryonic red cell metabolism.
FAU - Koller, M
AU  - Koller M
AD  - Physiologisches Institut, Universitat Regensburg, Germany.
FAU - Dragon, S
AU  - Dragon S
FAU - Baumann, R
AU  - Baumann R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Diphosphoglyceric Acids)
RN  - 0 (Enzymes)
RN  - 0 (Nucleotides)
RN  - 138-81-8 (2,3-Diphosphoglycerate)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - 2,3-Diphosphoglycerate
MH  - Adenine Nucleotides/metabolism
MH  - Adenosine Monophosphate/*metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Carbonic Anhydrases/biosynthesis
MH  - Chick Embryo
MH  - Diphosphoglyceric Acids/metabolism
MH  - Enzymes/metabolism
MH  - Erythrocytes/*physiology
MH  - Extracellular Space/*metabolism
MH  - *Fetal Blood
MH  - Nucleotides/metabolism
MH  - Ovum/*physiology
MH  - Oxygen/blood
MH  - Plasma/physiology
MH  - Time Factors
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1152/ajpregu.1994.267.2.R542 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Aug;267(2 Pt 2):R542-8. doi: 10.1152/ajpregu.1994.267.2.R542.