PMID- 8069309
OWN - NLM
STAT- MEDLINE
DCOM- 19940927
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 3
IP  - 4
DP  - 1994 Apr
TI  - High resolution ordering of YAC contigs using extended chromatin and chromosomes.
PG  - 629-33
AB  - The general usefulness of fluorescence in situ hybridization (FISH) for the 
      physical mapping of the human genome is greatly enhanced by improved DNA 
      resolution. Several techniques have been described for decondensing or stretching 
      interphase chromatin in a linear fashion, allowing long-range FISH mapping in 
      finer detail. Highly extended linear chromatin can be hybridized over physical 
      distances of at least several megabases, possibly over the whole length of a 
      chromosome. By multi-color FISH, we have determined the order and overlaps of 
      YACs from a 5q34-35 contig. Cosmids can be localized within larger YAC clones. 
      Extended chromatin mapping can be applied as an adjunct ordering technique in 
      genome studies.
FAU - Haaf, T
AU  - Haaf T
AD  - Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
FAU - Ward, D C
AU  - Ward DC
LA  - eng
GR  - HG-00246/HG/NHGRI NIH HHS/United States
GR  - HG-00272/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Chromatin)
RN  - 00DPD30SOY (Amsacrine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amsacrine/pharmacology
MH  - Chromatin/drug effects/*ultrastructure
MH  - Chromosome Mapping/*methods
MH  - *Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human/drug effects/ultrastructure
MH  - Chromosomes, Human, Pair 5/ultrastructure
MH  - Cosmids
MH  - DNA/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Spermatozoa/chemistry/ultrastructure
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1093/hmg/3.4.629 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1994 Apr;3(4):629-33. doi: 10.1093/hmg/3.4.629.