PMID- 8070617
OWN - NLM
STAT- MEDLINE
DCOM- 19940923
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 43
IP  - 9
DP  - 1994 Sep
TI  - Opposite effects of beta-cell differentiation and growth on reg expression in 
      human fetal pancreatic cells.
PG  - 1164-6
AB  - Reg is a gene associated with regeneration of pancreatic islets. We have 
      previously shown that nicotinamide induces differentiation of human fetal 
      beta-cells in tissue culture and that hepatocyte growth factor/scatter factor 
      (HGF/SF) is mitogenic for the fetal beta-cells. We now tested whether these 
      conditions, supporting either differentiation or growth, are associated with 
      changes in reg gene expression in human fetal pancreatic cells. Culture for 7 
      days with 10 mM nicotinamide led to a fourfold decrease in reg mRNA levels (23 
      +/- 12% of control, n = 5, P < 0.001). In contrast, HGF/SF increased reg 
      expression threefold (302 +/- 68% of control, n = 4, P < 0.05). Nicotinamide, 
      which does not alter the differentiation level of adult beta-cells, did not 
      significantly affect reg expression in adult human islets (84 +/- 4% of control, 
      n = 2, NS). Thus, a higher level of endocrine differentiation is associated with 
      a lower level of reg expression, and a higher rate of beta-cell replication 
      results in increased reg transcription. These results provide the first evidence 
      of a molecular marker, the reg gene, to distinguish between proliferation and 
      differentiation of human beta-cells.
FAU - Otonkoski, T
AU  - Otonkoski T
AD  - Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92037.
FAU - Mally, M I
AU  - Mally MI
FAU - Hayek, A
AU  - Hayek A
LA  - eng
GR  - R01-DK-39087/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Lithostathine)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (REG1A protein, human)
RN  - 0 (RNA, Messenger)
RN  - 25X51I8RD4 (Niacinamide)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 5.1.1.- (Amino Acid Isomerases)
RN  - EC 5.2.1.8 (Peptidylprolyl Isomerase)
SB  - IM
GS  - reg
MH  - Amino Acid Isomerases/biosynthesis
MH  - Calcium-Binding Proteins/*biosynthesis
MH  - Carrier Proteins/biosynthesis
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Endodeoxyribonucleases/biosynthesis
MH  - Fetus
MH  - *Gene Expression
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Islets of Langerhans/*cytology/drug effects/metabolism
MH  - Lithostathine
MH  - *Nerve Tissue Proteins
MH  - Niacinamide/pharmacology
MH  - Peptidylprolyl Isomerase
MH  - Phosphoproteins/biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Transcription, Genetic
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.2337/diab.43.9.1164 [doi]
PST - ppublish
SO  - Diabetes. 1994 Sep;43(9):1164-6. doi: 10.2337/diab.43.9.1164.