PMID- 8074246
OWN - NLM
STAT- MEDLINE
DCOM- 19940923
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 2 Pt 1
DP  - 1994 Aug
TI  - Passive sensitization of human bronchi augments smooth muscle shortening velocity 
      and capacity.
PG  - L218-22
AB  - We assessed whether incubation with human serum from atopic individuals 
      containing high concentrations of immunoglobulin E (IgE) causes augmentation of 
      maximal contraction of human bronchial smooth muscle from non-atopic subjects in 
      vitro. Bronchi were obtained from eight patients undergoing lung resection, and 
      force-velocity relationships were determined for eight pairs of epithelium-intact 
      bronchial rings of generations 6-7 using an electromagnetic lever system, which 
      allowed isotonic shortening when load-clamps [from 0 to maximal isometric force 
      (P0)] were applied at specific times after onset of contraction. Contractions 
      were elicited by supramaximal electrical field stimulation (50 Hz, 10 s train 
      duration, 25 V). Optimal length (Lo) for each tissue was determined during 
      equilibration. After resection, tissues were sensitized passively with human sera 
      containing high titers (> 1,000 U/ml) of IgE by incubation for 16 h at 20 degrees 
      C. Maximal shortening velocity (Vmax) was increased for passively sensitized 
      bronchi [0.1150 +/- 0.0240 1/2 circumferences/s (1/2Cir/s)] compared with 
      sham-sensitized bronchi [0.0731 +/- 0.0152 1/2Cir/s, P = 0.038]. Similarly, 
      maximal shortening (delta Lmax) was augmented in sensitized bronchial rings 
      (11.27 +/- 1.80 %Lo) compared with sham-sensitized tissues (8.19 +/- 1.39 %Lo, P 
      = 0.012). However, P0 did not differ between sensitized (122.5 +/- 24.4 mN/cm2) 
      compared with sham-sensitized tissues (138.4 +/- 32.1 mN/cm2, P = 0.642). Our 
      data are the first demonstration that Vmax and delta Lmax are augmented in 
      sensitized but not challenged human bronchial rings after passive sensitization 
      using human serum containing high concentrations of IgE.
FAU - Mitchell, R W
AU  - Mitchell RW
AD  - Asthma Allergy and Immunology Disease Research Center, University of Chicago, 
      Illinois 60637.
FAU - Ruhlmann, E
AU  - Ruhlmann E
FAU - Magnussen, H
AU  - Magnussen H
FAU - Leff, A R
AU  - Leff AR
FAU - Rabe, K F
AU  - Rabe KF
LA  - eng
GR  - HL-35718/HL/NHLBI NIH HHS/United States
GR  - HL-46368/HL/NHLBI NIH HHS/United States
GR  - UO1-34566/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
SB  - IM
MH  - Bronchi/*immunology/*physiology
MH  - *Bronchoconstriction
MH  - Female
MH  - Humans
MH  - *Immunization, Passive
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth/*physiology
MH  - Time Factors
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1152/ajplung.1994.267.2.L218 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Aug;267(2 Pt 1):L218-22. doi: 10.1152/ajplung.1994.267.2.L218.