PMID- 8074665
OWN - NLM
STAT- MEDLINE
DCOM- 19940929
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 203
IP  - 1
DP  - 1994 Aug 30
TI  - Involvement of Fc epsilon RII/CD23 and L-arginine dependent pathway in 
      IgE-mediated activation of human eosinophils.
PG  - 265-71
AB  - Eosinophils display various receptors for immunoglobulin E (IgE) including the 
      high affinity receptor for IgE (Fc epsilon RI), CD23 (Fc epsilon RII), and 
      Mac-2/epsilon BP. We attempted here to clarify the role of these receptors in 
      IgE-mediated activation of eosinophils from normal human bone marrow cultures. 
      Pretreatment of eosinophils with IL-4 is required for IgE/anti-IgE-mediated 
      stimulation of TNF-alpha and peroxydes production. TNF-alpha release from 
      eosinophils was also induced following ligation of CD23 and to a lesser extent 
      with anti-Mac-2, while Fc epsilon RI-ligation had no effect. IgE/anti-IgE effect 
      dramatically decreased when eosinophils were pretreated with Fab fragments of 
      CD23-mAb. In addition, this effect could also be reversed by inhibiting 
      CD23-dependent nitric oxide pathway by NG-monomethyl-L-arginine. Nitric oxide 
      chemical donor, SIN-1, induced TNF-alpha release from eosinophils. CD23 and 
      nitric oxide pathway are thus involved in IgE-mediated stimulation of normodense 
      human eosinophils.
FAU - Arock, M
AU  - Arock M
AD  - Groupe d'Immuno-Hematologie Moleculaire, CNRS URA625, CHU Pitie-Salpetriere, 
      Paris, France.
FAU - Le Goff, L
AU  - Le Goff L
FAU - Becherel, P A
AU  - Becherel PA
FAU - Dugas, B
AU  - Dugas B
FAU - Debre, P
AU  - Debre P
FAU - Mossalayi, M D
AU  - Mossalayi MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Peroxides)
RN  - 0 (Receptors, IgE)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11062-77-4 (Superoxides)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 5O5U71P6VQ (linsidomine)
RN  - 94ZLA3W45F (Arginine)
RN  - D46583G77X (Molsidomine)
SB  - IM
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antihypertensive Agents/pharmacology
MH  - Arginine/*analogs & derivatives/*metabolism/pharmacology
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eosinophils/drug effects/immunology/*physiology
MH  - Hematopoietic Stem Cells/drug effects/immunology/*physiology
MH  - Humans
MH  - Immunoglobulin E/*pharmacology
MH  - Kinetics
MH  - Molsidomine/analogs & derivatives/pharmacology
MH  - Peroxides/metabolism
MH  - Receptors, IgE/drug effects/immunology/*physiology
MH  - Superoxides/metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
MH  - omega-N-Methylarginine
EDAT- 1994/08/30 00:00
MHDA- 1994/08/30 00:01
CRDT- 1994/08/30 00:00
PHST- 1994/08/30 00:00 [pubmed]
PHST- 1994/08/30 00:01 [medline]
PHST- 1994/08/30 00:00 [entrez]
AID - S0006291X84721772 [pii]
AID - 10.1006/bbrc.1994.2177 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Aug 30;203(1):265-71. doi: 
      10.1006/bbrc.1994.2177.