PMID- 8075423
OWN - NLM
STAT- MEDLINE
DCOM- 19940930
LR  - 20240109
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 7
IP  - 4
DP  - 1994 Jul-Aug
TI  - Expression and localization of two in planta induced extracellular proteins of 
      the fungal tomato pathogen Cladosporium fulvum.
PG  - 516-24
AB  - Expression of two in planta induced genes of the biotrophic fungal tomato 
      pathogen Cladosporium fulvum and accumulation of their products, extracellular 
      proteins (ECPs), were studied in time and space during pathogenesis. Immunogold 
      localization revealed that proteins ECP1 and ECP2 accumulated abundantly in 
      extracellular material in the vicinity of fungal and host cell walls. Expression 
      of the genes encoding ECP1 and ECP2 was studied in transformants carrying the 
      reporter gene Gus fused to promoter sequences of the ecp genes. In germinated 
      conidia on the leaf surface no expression of gene ecp1 and only low expression of 
      gene ecp2 could be detected. Expression of both ecp1 and ecp2 was strongly 
      induced during colonization of the intercellular space between tomato mesophyll 
      cells. The highest expression was observed in hyphae growing near vascular 
      tissue. Expression levels were low in newly formed conidia on leaves. Possible 
      functions of ECP1 and ECP2 for C. fulvum during pathogenesis are discussed.
FAU - Wubben, J P
AU  - Wubben JP
AD  - Department of Phytopathology, Agricultural University, Wageningen, The 
      Netherlands.
FAU - Joosten, M H
AU  - Joosten MH
FAU - De Wit, P J
AU  - De Wit PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - 0 (ECP1 protein, Cladosporium fulvum)
RN  - 0 (ECP2 protein, Cladosporium fulvum)
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Base Sequence
MH  - Cladosporium/growth & development/*metabolism
MH  - Fungal Proteins/*biosynthesis/isolation & purification
MH  - Genes, Fungal/genetics
MH  - Genes, Reporter
MH  - Glucuronidase/biosynthesis/genetics
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Plant Diseases/*microbiology
MH  - Recombinant Fusion Proteins/biosynthesis/isolation & purification
MH  - Vegetables/*microbiology
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1094/mpmi-7-0516 [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 1994 Jul-Aug;7(4):516-24. doi: 10.1094/mpmi-7-0516.