PMID- 8076354
OWN - NLM
STAT- MEDLINE
DCOM- 19941004
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 76
IP  - 1
DP  - 1994 Aug
TI  - Detection of minimal residual disease state in chronic myelogenous leukemia 
      patients using fluorescence in situ hybridization.
PG  - 59-64
AB  - Detection of minimal residual disease and relapse remain major problems in 
      chronic myelogenous leukemia (CML) patients following bone marrow transplantation 
      (BMT). In order to disclose the 9;22 Philadelphia translocation, we used a 
      fluorescence in situ hybridization (FISH) technique. BCR and ABL gene fragments 
      were used as probes for the detection of the BCR/ABL fusion product in peripheral 
      blood and bone marrow cells from 11 CML patients in which 5 were post-BMT. The 
      sensitivity and specificity of this approach were compared to conventional 
      cytogenetic and polymerase chain reaction (PCR) methods. FISH demonstrated a high 
      degree of sensitivity (1%) for the detection of the BCR/ABL translocation in 
      these patients. A linear correlation was found between FISH detection of the 
      BCR/ABL fusion product and routine chromosomal analysis (r = 0.995; p < 0.001). 
      Detection of the BCR/ABL signal by FISH was observed in all patients showing a 
      positive PCR signal. A significant reduction in BCR/ABL signal was observed 
      post-transplant (p < 0.001). However, the BCR/ABL translocation was detected in 
      four of five transplanted patients immediately (0.75-2.5 months) following 
      transplant and was found in patients with a low expression of the translocation.
FAU - Amiel, A
AU  - Amiel A
AD  - Cytogenetics Unit, Meir General Hospital, Kfar-Saba, Israel.
FAU - Yarkoni, S
AU  - Yarkoni S
FAU - Slavin, S
AU  - Slavin S
FAU - Or, R
AU  - Or R
FAU - Lorberboum-Galski, H
AU  - Lorberboum-Galski H
FAU - Fejgin, M
AU  - Fejgin M
FAU - Nagler, A
AU  - Nagler A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Bone Marrow Transplantation
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Translocation, Genetic
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 0165-4608(94)90073-6 [pii]
AID - 10.1016/0165-4608(94)90073-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Aug;76(1):59-64. doi: 10.1016/0165-4608(94)90073-6.