PMID- 8078302
OWN - NLM
STAT- MEDLINE
DCOM- 19940930
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 71
IP  - 2
DP  - 1994 Aug
TI  - Expression of monocyte chemoattractant protein-1 in delayed type hypersensitivity 
      reactions in the skin.
PG  - 226-35
AB  - BACKGROUND: Monocyte chemoattractant Protein-1 (MCP-1) is a potent chemotactic 
      factor for monocytes. Because many inflammatory dermatoses are characterized by 
      mononuclear cell infiltrates, it is reasonable to postulate that MCP-1 might be 
      involved in their pathogenesis. To date, no in vivo studies have been published 
      concerning the expression of MCP-1 in this context. The aim of this study was to 
      elucidate the expression of MCP-1 in human inflammatory skin diseases which are 
      thought to involve delayed type hypersensitivity reactions. EXPERIMENTAL DESIGN: 
      Expression of MCP-1 was examined in normal skin and three classes of inflammatory 
      skin reactions by immunohistochemistry experiments utilizing a monospecific MCP-1 
      antiserum. The distribution of monocytes/macrophages and T lymphocytes was 
      determined by immunohistochemistry using antibodies to specific cell surface 
      markers. RESULTS: Immunostaining with MCP-1 antiserum demonstrated strong MCP-1 
      expression in lichenoid dermatitis, dermal hypersensitivity reactions, and 
      spongiotic dermatitis. In contrast, normal skin showed minimal MCP-1 expression 
      in the dermis. The cell types displaying MCP-1 expression were endothelial cells 
      of dermal microvessels that were surrounded by lymphocytic infiltrates and 
      monocytes/macrophages at the periphery of the perivascular infiltrates. 
      Occasionally, MCP-1-positive mononuclear cells were present both in the 
      infiltrates and in a diffuse pattern in the surrounding dermis. Keratinocytes 
      were found to produce MCP-1 constitutively in normal skin and in inflamed 
      conditions. The pattern of MCP-1 expression was similar to the pattern observed 
      for monocyte/macrophage distribution, whereas the pattern of MCP-1 expression was 
      different from the pattern of T lymphocyte distribution. CONCLUSIONS: We observed 
      an enhanced expression of MCP-1 in inflammatory skin conditions. The expression 
      of MCP-1 provides a mechanistic explanation for the increased recruitment of 
      monocytes/macrophages in cell-mediated immune response such as delayed type 
      hypersensitivity reactions in the skin.
FAU - Yu, X
AU  - Yu X
AD  - Department of Oral Biology, Boston University School of Graduate Dentistry, 
      Massachusetts.
FAU - Barnhill, R L
AU  - Barnhill RL
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - DE07559/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
SB  - IM
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*metabolism
MH  - Cytokines/metabolism
MH  - Dermatitis/metabolism
MH  - Epidermis/metabolism
MH  - Humans
MH  - Hypersensitivity, Delayed/*metabolism
MH  - Phagocytes/metabolism
MH  - Reference Values
MH  - Skin/*immunology/*metabolism
MH  - T-Lymphocytes/metabolism
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1994 Aug;71(2):226-35.