PMID- 8080044
OWN - NLM
STAT- MEDLINE
DCOM- 19941004
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 145
IP  - 3
DP  - 1994 Sep
TI  - Sensitive detection of chromosome copy number aberrations in prostate cancer by 
      fluorescence in situ hybridization.
PG  - 624-30
AB  - The pattern of chromosomal aberrations and their significance in prostate cancer 
      are poorly understood. We studied 23 prostate cancer and 10 benign prostatic 
      hyperplasia (BPH) specimens by fluorescence in situ hybridization (FISH) using 
      pericentromeric repeat-specific probes for 10 different chromosomes. The aims of 
      the study were: 1) to compare the sensitivity of FISH and DNA flow cytometry in 
      aneuploidy detection, 2) to determine which chromosome copy number changes are 
      most common, and 3) which probe combinations would be most effective in 
      aneuploidy diagnosis. Disaggregated tumor cells from formalin-fixed, 
      paraffin-embedded tissues were pretreated with our newly developed method based 
      on hot glycerol solution to improve probe penetration. All BPH specimens were 
      diploid by DNA flow cytometry and showed no numerical chromosome aberrations by 
      FISH. In prostate cancer, flow cytometry showed abnormal DNA content in 35% of 
      cases, whereas 74% were abnormal by FISH. Aberrant copy number of chromosomes 8 
      (48% of cases), X (43% of cases), and 7 (39% of cases) were most common. 
      Ninety-four percent of all aneuploid cases would have been detected with these 
      three probes alone. Simple chromosome losses were uncommon but in DNA tetraploid 
      tumors relative losses (trisomy or disomy) of several chromosomes were often 
      found, suggesting progression of prostate cancer through tetraploidization 
      followed by losses of selected chromosomes. In conclusion, our results indicate 
      that FISH using three selected chromosome-specific probes is two to three times 
      more sensitive than flow cytometric DNA content analysis in aneuploidy detection.
FAU - Visakorpi, T
AU  - Visakorpi T
AD  - Department of Clinical Chemistry, Tampere University Hospital, Finland.
FAU - Hyytinen, E
AU  - Hyytinen E
FAU - Kallioniemi, A
AU  - Kallioniemi A
FAU - Isola, J
AU  - Isola J
FAU - Kallioniemi, O P
AU  - Kallioniemi OP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aneuploidy
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Disorders
MH  - *Chromosomes, Human, Pair 7
MH  - *Chromosomes, Human, Pair 8
MH  - DNA, Neoplasm
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Sensitivity and Specificity
MH  - *X Chromosome
PMC - PMC1890337
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
PMCR- 1995/03/01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PHST- 1995/03/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1994 Sep;145(3):624-30.