PMID- 8080653
OWN - NLM
STAT- MEDLINE
DCOM- 19941011
LR  - 20041117
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 38
IP  - 2
DP  - 1994 Jun
TI  - Analysis of mRNA for class I HLA on human gametogenic cells.
PG  - 231-7
AB  - We have studied mRNA expression for Class I HLA (human leukocyte antigen) on male 
      germ cells by amplification of gene fragments in PCR technique and by Northern 
      hybridization. RNA was extracted from fractionated gametogenic cells (isolated 
      from testis) and reversely transcribed. Then, cDNA was amplified for specific HLA 
      sequence (HLA, -A, -B, -C). The specificity of this product was confirmed in 
      "nested" PCR of 400 bp gene fragment coding for alpha 2 domain, alpha 3 domain, 
      and the transmembrane portion of Class I HLA. The results indicate minimal 
      expression of classical Class I HLA on gametogenic cells. Northern hybridization 
      with 669 bp cDNA fragment (spanning for alpha 3 domain, transmembrane, 
      cytoplasmic, and 3' untranslated region) resulted in a low intensity signal from 
      gametogenic cell fractions and confirmed our findings obtained by PCR. The 
      minimal expression of classical HLA antigens may create a neutral cover for the 
      male reproductive system, thereby preventing an immunological response during 
      germ cell differentiation.
FAU - Janitz, M
AU  - Janitz M
AD  - Institute of Human Genetics, Polish Academy of Sciences, Poznan.
FAU - Fiszer, D
AU  - Fiszer D
FAU - Michalczak-Janitz, K
AU  - Michalczak-Janitz K
FAU - Lukaszyk, A
AU  - Lukaszyk A
FAU - Fernandez, N
AU  - Fernandez N
FAU - Skorupski, W
AU  - Skorupski W
FAU - Kurpisz, M
AU  - Kurpisz M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA, Complementary)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Blotting, Northern
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - HLA Antigens/*genetics
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Male
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*analysis/*genetics
MH  - Spermatids/chemistry/immunology
MH  - Spermatozoa/chemistry/*immunology
MH  - Testis/chemistry/cytology/immunology
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1002/mrd.1080380215 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1994 Jun;38(2):231-7. doi: 10.1002/mrd.1080380215.