PMID- 8081355
OWN - NLM
STAT- MEDLINE
DCOM- 19941011
LR  - 20220316
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 3
IP  - 5
DP  - 1994 May
TI  - Structural analysis of alpha-satellite DNA and centromere proteins using extended 
      chromatin and chromosomes.
PG  - 697-709
AB  - Human centromeres are characterized by distinct subsets of alpha-satellite DNA 
      and by a number of centromeric proteins (CENPs) at least one of which, CENP-B, 
      binds specifically to alpha-satellite DNA sequences. When the centromeres of 
      metaphase chromosomes are mechanically stretched to five to 20 times their normal 
      length, CENPs specifically recognized by CREST autoantibodies extend over the 
      entire length of the linear alpha-satellite array. For higher resolution analysis 
      we spread interphase chromatin across a slide resulting in highly extended 
      chromatin fibers. By fluorescence in situ hybridization (FISH) with human 
      alpha-satellite DNA and an oligomer specific for the CENP-B box sequence, the 
      regular spacing of CENP-B binding motifs within arrays of alpha-satellite DNA was 
      visualized directly. FISH with elongated chromatin structures released from 
      interphase nuclei with the drug 
      N-[4-(9-acridinylamino)-3-methoxyphenyl]methanesulfonamide shows that D7Z1 and 
      D7Z2, two distinct alpha-satellite arrays on chromosome 7, are not interspersed 
      with each other but are separated by as little as several hundred kilobases, 
      consistent with previous long-range mapping data. The D7Z2 array, which does not 
      bind detectable amounts of CENPs, can be assigned to the short arm side of the 
      D7Z1 array using artificially stretched chromosomes. In interphase nuclei 
      unreplicated segments give a singlet hybridization signal, whereas fully 
      replicated loci appear as doublets. Although D7Z1 is replicated prior to D7Z2 in 
      the majority of cells, the replication timing of one array relative to the other 
      is variable. The replication of alpha-satellite arrays on homologous chromosomes 
      is highly asynchronous. The newly replicated alpha-satellite lacks the CENP 
      component.
FAU - Haaf, T
AU  - Haaf T
AD  - Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
FAU - Ward, D C
AU  - Ward DC
LA  - eng
GR  - HG-00246/HG/NHGRI NIH HHS/United States
GR  - HG-00272/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Autoantigens)
RN  - 0 (CENPB protein, human)
RN  - 0 (Centromere Protein B)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Satellite)
RN  - 0 (DNA-Binding Proteins)
RN  - 00DPD30SOY (Amsacrine)
SB  - IM
MH  - Amsacrine/pharmacology
MH  - Antibodies, Antinuclear/immunology
MH  - Autoantigens/analysis/immunology
MH  - CREST Syndrome/immunology
MH  - Centromere/*chemistry/immunology
MH  - Centromere Protein B
MH  - Chromosomal Proteins, Non-Histone/*analysis/immunology
MH  - Chromosome Mapping
MH  - Chromosomes, Human/chemistry/drug effects/*ultrastructure
MH  - DNA Replication
MH  - DNA, Satellite/*analysis/biosynthesis
MH  - *DNA-Binding Proteins
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphocytes/ultrastructure
MH  - Male
MH  - Metaphase
MH  - Repetitive Sequences, Nucleic Acid
MH  - Spermatozoa/ultrastructure
MH  - Stress, Mechanical
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1093/hmg/3.5.697 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1994 May;3(5):697-709. doi: 10.1093/hmg/3.5.697.