PMID- 8083459
OWN - NLM
STAT- MEDLINE
DCOM- 19941013
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 94
IP  - 3 Pt 1
DP  - 1994 Sep
TI  - Ultrastructural immunogold localization of tumor necrosis factor-alpha to the 
      cytoplasmic granules of rat peritoneal mast cells with rapid microwave fixation.
PG  - 531-6
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional, proinflammatory 
      cytokine, which can be produced by mast cells and several other cell types. We 
      used a newly developed microwave energy-assisted aldehyde fixation method to 
      prepare purified rat peritoneal mast cells for the postembedding immunogold 
      ultrastructural localization of TNF-alpha. These fixation methods were superior 
      to chemical fixation alone in preserving both the ultrastructural morphology and 
      immunoreactive TNF-alpha in rat mast cells. The percent of TNF-alpha-positive 
      mast cells in samples prepared with microwave-assisted fixation in low (84%) and 
      standard (81%) glutaraldehyde concentrations exceeded that for low (56%) and 
      standard (15%) glutaraldehyde concentrations without the assistance of microwave 
      energy. TNF-alpha was identified in the large storage granules of rat mast cells. 
      The percent of positive granules in microwave-assisted standard (44%) and low 
      (40%) glutaraldehyde samples was considerably higher than the percent of positive 
      granules in standard (5%) and low (10%) glutaraldehyde-fixed samples without 
      microwave assistance. This location of TNF-alpha in rat peritoneal mast cells 
      suggests that this cytokine can use the regulated secretory route(s) for release 
      from appropriately stimulated rat mast cells into the microenvironment.
FAU - Beil, W J
AU  - Beil WJ
AD  - Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
FAU - Login, G R
AU  - Login GR
FAU - Galli, S J
AU  - Galli SJ
FAU - Dvorak, A M
AU  - Dvorak AM
LA  - eng
GR  - AI22674/AI/NIAID NIH HHS/United States
GR  - AI33372/AI/NIAID NIH HHS/United States
GR  - DE-10059/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cytoplasmic Granules/*chemistry/ultrastructure
MH  - *Immunohistochemistry
MH  - Male
MH  - Mast Cells/*chemistry/ultrastructure
MH  - Microscopy, Immunoelectron
MH  - Microwaves
MH  - Peritoneal Cavity/cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Fixation/*methods
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - S0091674994000503 [pii]
AID - 10.1016/0091-6749(94)90210-0 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 1994 Sep;94(3 Pt 1):531-6. doi: 
      10.1016/0091-6749(94)90210-0.