PMID- 8083688
OWN - NLM
STAT- MEDLINE
DCOM- 19941010
LR  - 20190512
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 53
IP  - 5
DP  - 1994 Sep
TI  - Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies 
      in normal and gliotic brain.
PG  - 448-56
AB  - In vitro tissue culturing, for karyotype analysis, may introduce artifacts 
      confounding the cytogenetic evaluation of tissues with low baseline proliferative 
      activity. Utilizing a panel of fluorescence in situ hybridization (FISH) probes 
      for chromosomes 7, 8, 9, 10, 12, 17, 18, X, and Y, we compared the results of 
      FISH analysis of non-tumorous normal (11 patients) and gliotic (10 patients) 
      brain tissue touch preparations with those of cytogenetic evaluation performed on 
      short-term primary cultures of the same material. We found a significant rate of 
      apparent monosomy of chromosomes 8 and 17 by FISH analysis, with no corresponding 
      clonal chromosomal loss detected by karyotype evaluation. These monosomy rates 
      were significantly lower in gliotic than in normal brain tissue, and image 
      analysis suggested that this apparent monosomy was due to interphase pairing of 
      homologous centromere signals. Two distinct Y-chromosome signals were seen in 
      9.4% of nuclei by FISH, with 3 of 15 males displaying disomy Y rates over 15%. 
      Disomy Y rates correlated approximately with age and clonal disomy Y was seen in 
      the karyotype of one of these specimens. Karyotype analysis demonstrated loss of 
      a sex chromosome in 6 specimens, while no sex chromosome nullisomy was detected 
      by FISH. FISH is a valuable adjunct to the cytogenetic evaluation of tissues with 
      low baseline proliferative activity. The differences in relative monosomy rates 
      between normal and gliotic brain suggest that alterations in nuclear architecture 
      and/or DNA sequence accompany the transition from normal to reactive glia.
FAU - Dalrymple, S J
AU  - Dalrymple SJ
AD  - Department of Neurologic Surgery, Mayo Medical Center, Rochester, Minnesota.
FAU - Herath, J F
AU  - Herath JF
FAU - Borell, T J
AU  - Borell TJ
FAU - Moertel, C A
AU  - Moertel CA
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cytogenetics
MH  - Female
MH  - Gliosis/*genetics
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Monosomy/genetics
MH  - Reference Values
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1097/00005072-199409000-00004 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 1994 Sep;53(5):448-56. doi: 
      10.1097/00005072-199409000-00004.