PMID- 8084308
OWN - NLM
STAT- MEDLINE
DCOM- 19941010
LR  - 20190904
IS  - 0098-1532 (Print)
IS  - 0098-1532 (Linking)
VI  - 23
IP  - 5
DP  - 1994
TI  - Interphase cytogenetic study of childhood acute lymphoblastic leukemia.
PG  - 413-21
AB  - We used the fluorescence in situ hybridization (FISH) technique and 
      centromere-specific probes for chromosomes 1, 6, 8, 10, 12, 17, 18, X, and Y to 
      investigate the presence and number of the respective chromosomes in interphase 
      nuclei of 14 cases of childhood acute lymphoblastic leukemia (ALL) which were 
      shown to be hyperdiploid by DNA flow cytometry irrespective of their cytogenetic 
      pattern. Numerical anomalies for one or more chromosomes were detected in all 14 
      cases. The FISH results were compared with those obtained by conventional 
      cytogenetic analysis. A hyperdiploid karyotype was evident in 5 cases, the others 
      were either normal or lacking cytogenetic results because of technical failure. 
      In the 5 cytogenetically hyperdiploid cases, 14 numerical abnormalities were 
      observed with both techniques, whereas 4 numerical deviations were found only 
      with FISH. In 9 other cases which had a DNA content indicating hyperdiploidy, 34 
      trisomies and 2 tetrasomies were detected by FISH analysis. Furthermore, in 1 
      case duplication of the Y chromosome and in 3 male cases duplication of the X 
      chromosome were evident. Double-target FISH experiments in 2 patients allowed the 
      correlation of numerical aberrations of 2 chromosomes in one and the same cell. 
      By such analyses, detection of subpopulations of tumor cells was found to be 
      relatively easy. Our results indicate that the FISH technique with 
      chromosome-specific repetitive centromeric probes is a rapid, simple to use, and 
      easy to interpret technique for the evaluation of numerical chromosomal 
      aberrations in interphase nuclei of leukemias.
FAU - Berger, A
AU  - Berger A
AD  - Children's Cancer Research Institute, St. Anna Children's Hospital, Vienna, 
      Austria.
FAU - Strehl, S
AU  - Strehl S
FAU - Hekele, A
AU  - Hekele A
FAU - Ambros, P F
AU  - Ambros PF
FAU - Haas, O A
AU  - Haas OA
FAU - Gadner, H
AU  - Gadner H
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Med Pediatr Oncol
JT  - Medical and pediatric oncology
JID - 7506654
SB  - IM
MH  - *Aneuploidy
MH  - Child
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/mpo.2950230505 [doi]
PST - ppublish
SO  - Med Pediatr Oncol. 1994;23(5):413-21. doi: 10.1002/mpo.2950230505.