PMID- 8084616
OWN - NLM
STAT- MEDLINE
DCOM- 19941013
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 9
IP  - 10
DP  - 1994 Oct
TI  - Deletion mapping of chromosome 8p in prostate cancer by fluorescence in situ 
      hybridization.
PG  - 3071-6
AB  - Double-target fluorescence in situ hybridization (FISH) was applied to 42 cases 
      of prostate cancer and seven cases of histologically proven benign prostate 
      hyperplasia for the detection of structural aberrations of chromosome 8. Cosmid 
      probes for two chromosome 8p loci (LPL/8p22 and D8S7/8p23) were used in 34 
      specimens of malignant tumors obtained by the touch biopsy technique. Deletion 
      was defined as when the number of cosmid signals was lower than the number of 
      centromere signals in more than 35% of all nuclei observed. In total, thirty of 
      the 42 (71%) specimens demonstrated any type of 8p deletion. Out of the 34 cases 
      in which deletion mapping could be evaluated, distal deletion (D8S7) was detected 
      in 17 (50%), of which 10 also showed deletion of LPL. Deletion of LPL was 
      detected in 18 cases (53%), of which 8 (24%) retained the D8S7 (interstitial 
      deletion). When the deletion pattern was graded as (1) no deletion (2) partial 
      deletion (either D8S7 or LPL deleted) and (3) both deletions, the degree of 
      deletion was well correlated with the tumor grade (P = 0.0009) and with stage (P 
      = 0.0072, Fisher's Exact test). These data support the hypothesis that tumor 
      suppressor gene(s) may be located in the chromosomal region 8p22, hence 8p 
      deletions may play a crucial role in the pathogenesis of prostate cancer.
FAU - Matsuyama, H
AU  - Matsuyama H
AD  - Department of Urology, Karolinska Hospital, Stockholm, Sweden.
FAU - Pan, Y
AU  - Pan Y
FAU - Skoog, L
AU  - Skoog L
FAU - Tribukait, B
AU  - Tribukait B
FAU - Naito, K
AU  - Naito K
FAU - Ekman, P
AU  - Ekman P
FAU - Lichter, P
AU  - Lichter P
FAU - Bergerheim, U S
AU  - Bergerheim US
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Prostatic Neoplasms/*genetics/pathology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1994 Oct;9(10):3071-6.