PMID- 8086478
OWN - NLM
STAT- MEDLINE
DCOM- 19941020
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1219
IP  - 1
DP  - 1994 Sep 13
TI  - Binding of AP-1/CREB proteins and of MDBP to contiguous sites downstream of the 
      human TGF-beta 1 gene.
PG  - 55-63
AB  - Transcription of the human gene encoding transforming growth factor beta 1 
      (TGF-beta 1), which is a key regulator of cell growth and differentiation, is 
      inducible by phorbol esters. DNA sequences resembling phorbol ester response 
      elements (TREs) are present upstream and downstream of this gene. TREs are 
      recognized by proteins from the AP-1 family of transcription factors. We examined 
      a 16 basepair (bp) sequence downstream of the TGF-beta 1 gene that contains three 
      putative TREs. This sequence had been shown to stimulate reporter gene expression 
      from a downstream location in response to phorbol ester treatment. 
      Electrophoretic mobility shift assays suggest that minor proteins from the 
      related AP-1 and CREB families of transcription factors bind to the overlapping 
      TREs within the 16 bp element. A site beginning at the end of this 16 bp element 
      matches the consensus sequence of a DNA-binding protein called MDBP and was shown 
      to bind to this protein. When the intact MDBP site was present in a reporter gene 
      construct in addition to the TREs, the phorbol ester-induced stimulation of 
      reporter gene expression was no longer observed. This suggests that MDBP can 
      counteract the stimulation of transcription by AP-1/CREB-like proteins binding to 
      this downstream enhancer element.
FAU - Asiedu, C K
AU  - Asiedu CK
AD  - Department of Biochemistry SL43, Tulane Medical School, New Orleans, LA 70112.
FAU - Scotto, L
AU  - Scotto L
FAU - Assoian, R K
AU  - Assoian RK
FAU - Ehrlich, M
AU  - Ehrlich M
LA  - eng
GR  - GM33999/GM/NIGMS NIH HHS/United States
GR  - HL38884/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MBD1 protein, human)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Cyclic AMP Response Element-Binding Protein/classification/*metabolism
MH  - DNA/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Genes, Reporter
MH  - Humans
MH  - Methylation
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/metabolism
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-jun/classification/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Structure-Activity Relationship
MH  - Transcription Factors
MH  - Transcription, Genetic
MH  - Transforming Growth Factor beta/biosynthesis/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1994/09/13 00:00
MHDA- 1994/09/13 00:01
CRDT- 1994/09/13 00:00
PHST- 1994/09/13 00:00 [pubmed]
PHST- 1994/09/13 00:01 [medline]
PHST- 1994/09/13 00:00 [entrez]
AID - 0167-4781(94)90246-1 [pii]
AID - 10.1016/0167-4781(94)90246-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Sep 13;1219(1):55-63. doi: 
      10.1016/0167-4781(94)90246-1.