PMID- 8088377
OWN - NLM
STAT- MEDLINE
DCOM- 19941020
LR  - 20171116
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 22
IP  - 10
DP  - 1994 Sep
TI  - Enhancing effects of cyclosporin A on hematopoietic progenitors: possible role of 
      CD8+ T cells as negative regulators.
PG  - 947-53
AB  - To elucidate the mechanism by which the administration of cyclosporin A (Cy A) 
      improves a certain type of anemia, Cy A was orally administered to normal mice; 
      hematopoietic activity, natural killer (NK) cell activity, and natural suppressor 
      (NS) cell activity in the bone marrow (BM) were then examined. Hematopoietic 
      colony formation was significantly enhanced by both 7-day and 21-day treatment 
      with Cy A. Cy A did not, however, affect the total cell count or the numbers of 
      macrophages, granulocytes, or B cells in the BM. Neither did Cy A affect the in 
      vitro colony formation of the purified hematopoietic progenitors. These results 
      suggest that Cy A acts on the negative regulators such as NK cells, NS cells, and 
      CD8+ T cells. Since both NK and NS activity were also enhanced by Cy A treatment, 
      these cells were not candidates. CD4+ or CD8+ T cells in the thymus, peripheral 
      blood (PBL), and BM decreased as a result of the treatment. Therefore, to further 
      examine the involvement of CD4+ or CD8+ T cells, hematopoietic colony formation 
      was evaluated using bone marrow cells (BMCs) from mice purged of CD4+ and/or CD8+ 
      cells. A significant enhancement of erythroid and multipotent colonies was 
      observed in the BM of the CD8+ cell-purged mice. On the other hand, a significant 
      enhancement of myeloid colonies was found in the BM of the CD4+ cell-purged mice. 
      These findings suggest that single-positive T cells (particularly CD8+ T cells), 
      which are diminished in number after Cy A treatment, are involved in the negative 
      regulation of hematopoiesis.
FAU - Hashimoto, F
AU  - Hashimoto F
AD  - First Department of Pathology, Kansai Medical University, Osaka, Japan.
FAU - Sugiura, K
AU  - Sugiura K
FAU - Inoue, K
AU  - Inoue K
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (CD8 Antigens)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - CD8 Antigens/*analysis
MH  - Cyclosporine/*pharmacology
MH  - Erythroid Precursor Cells/drug effects
MH  - Female
MH  - Granulocytes/cytology
MH  - Hematopoietic Stem Cells/*drug effects
MH  - Killer Cells, Natural/immunology
MH  - Lymphocyte Depletion
MH  - Macrophages/cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Spleen/cytology
MH  - Stem Cells/drug effects
MH  - T-Lymphocytes/*immunology/*physiology
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1994 Sep;22(10):947-53.