PMID- 8088924
OWN - NLM
STAT- MEDLINE
DCOM- 19941019
LR  - 20190920
IS  - 0360-3997 (Print)
IS  - 0360-3997 (Linking)
VI  - 18
IP  - 3
DP  - 1994 Jun
TI  - Formation and mineralization of extracellular matrix secreted by an immortal 
      human osteoblastic cell line: modulation by tumor necrosis factor-alpha.
PG  - 267-84
AB  - Tumor necrosis factor-alpha (TNF-alpha), a 17-kDa cytokine produced by stimulated 
      macrophages/monocytes, modulates the functions of a variety of cells and has been 
      shown to induce bone resorption in vitro. However, the effects that TNF-alpha may 
      have on the process of bone formation are not completely understood. In order to 
      study the effects of TNF-alpha on matrix development and mineralization, we 
      utilized a human osteoblastic cell line, HOS TE85. Our results show that HOS 
      TE85, which has been shown to be responsive to hormones active on normal 
      osteoblasts, forms an extensive extracellular matrix (ECM) that mineralizes 
      during extended culture. Treatment during the development of the matrix with 
      TNF-alpha has little effect on cell number and DNA synthesis, showing thereby 
      that TNF-alpha is not cytotoxic to the cells. However, TNF-alpha inhibits the 
      formation of alkaline phosphatase (AP)-positive foci in a dose-dependent manner 
      at concentrations of 0.1-10 ng/ml. TNF-alpha treatment caused a significant 
      decrease in the incorporation of collagen into the developing matrix. In 
      addition, TNF-alpha treatment resulted in a significant decrease in the synthesis 
      of AP by HOS TE85 cells during the process of ECM formation and resulted in a 
      pronounced lack of mineralization of the ECM. These results indicate that 
      TNF-alpha may be acting as an uncoupler by decreasing the synthesis and 
      incorporation of proteins required for bone formation, and inhibiting matrix 
      formation and mineralization in vitro.
FAU - Panagakos, F S
AU  - Panagakos FS
AD  - Department of Biochemistry and Molecular Biology, University of Medicine and 
      Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
FAU - Hinojosa, L P
AU  - Hinojosa LP
FAU - Kumar, S
AU  - Kumar S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Minerals)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-34-5 (Collagen)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Cell Division/drug effects
MH  - Cell Line, Transformed
MH  - Collagen/metabolism
MH  - DNA/biosynthesis
MH  - Extracellular Matrix/metabolism/*physiology
MH  - Humans
MH  - Minerals/*metabolism
MH  - Osteoblasts/*metabolism/pathology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1007/BF01534268 [doi]
PST - ppublish
SO  - Inflammation. 1994 Jun;18(3):267-84. doi: 10.1007/BF01534268.