PMID- 8089806
OWN - NLM
STAT- MEDLINE
DCOM- 19941019
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 173
IP  - 2
DP  - 1994 Jun
TI  - Pathogenesis of IgA nephropathy: in vitro activation of human mesangial cells by 
      IgA immune complex leads to cytokine secretion.
PG  - 119-26
AB  - IgA immune complex (IC) plays a crucial role in the pathogenesis of IgA 
      nephropathy (IgAN). As IgA-IC is not itself cytotoxic, other mediators may be 
      involved in the pathogenesis. In order to elucidate the mechanisms by which 
      IgA-IC mediates renal injury in IgAN, the ability of IgA-IC to 'activate' 
      cultured human mesangial cells (HMC) was studied. HMC were incubated with 
      nephritogenic IgA-IC, containing a MOPC-315 plasmacytoma-derived IgA 
      anti-dinitrophenyl (DNP) and DNP-conjugated bovine serum albumin. The cells 
      showed morphological changes, an accelerated rate of proliferation, and increased 
      production of interleukin-1 (IL-1), interleukin-6 (IL-6), platelet activating 
      factor (PAF) and generation of superoxide anion. The enhancement of IL-1 and IL-6 
      mRNA expression in HMC incubated with IgA-IC was identified by dot blot analysis. 
      Northern blot hybridization also demonstrated an augmented IL-6 mRNA expression 
      in HMC treated with IgA-IC. These results suggest that nephritogenic IgA-IC may 
      amplify the proliferation of HMC and the production of immune/chemical mediators 
      and superoxide anion thereby resulting in the renal lesions of IgAN.
FAU - Chen, A
AU  - Chen A
AD  - Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, 
      Republic of China.
FAU - Chen, W P
AU  - Chen WP
FAU - Sheu, L F
AU  - Sheu LF
FAU - Lin, C Y
AU  - Lin CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukins)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (RNA, Messenger)
RN  - 11062-77-4 (Superoxides)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigen-Antibody Complex/*immunology
MH  - Blotting, Northern
MH  - Cell Division/immunology
MH  - Cells, Cultured
MH  - Child
MH  - Glomerular Mesangium/cytology/*immunology
MH  - Glomerulonephritis, IGA/*immunology
MH  - Humans
MH  - Immunoglobulin A/*immunology
MH  - Interleukin-1/biosynthesis/genetics
MH  - Interleukin-6/biosynthesis/genetics
MH  - Interleukins/*biosynthesis
MH  - Platelet Activating Factor/biosynthesis
MH  - RNA, Messenger/analysis
MH  - Superoxides/metabolism
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1002/path.1711730208 [doi]
PST - ppublish
SO  - J Pathol. 1994 Jun;173(2):119-26. doi: 10.1002/path.1711730208.