PMID- 8091661
OWN - NLM
STAT- MEDLINE
DCOM- 19941020
LR  - 20220330
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 204
IP  - 1
DP  - 1994 Oct
TI  - Human herpesvirus 6 (HHV-6) is a helper virus for adeno-associated virus type 2 
      (AAV-2) and the AAV-2 rep gene homologue in HHV-6 can mediate AAV-2 DNA 
      replication and regulate gene expression.
PG  - 304-11
AB  - We have previously described the apparent acquisition by human herpesvirus 6 
      (HHV-6) of the multifunctional rep gene of the helper-dependent human parvovirus 
      adeno-associated virus type 2 (AAV-2). We report here that HHV-6 is a full helper 
      virus for AAV-2 replication, suggesting a mechanism for transfer of the rep gene 
      between the two viruses by recombination of replicative intermediates. The HHV-6 
      rep gene cloned under control of the human cytomegalovirus immediate early 
      promoter complemented replication of a rep-deficient AAV-2 genome. In 
      cotransfection experiments with heterologous promoters linked to the CAT reporter 
      gene, HHV-6 rep activated the human immunodeficiency virus (HIV) long terminal 
      repeat (LTR) in fibroblast cell lines but not in T-cells. In contrast, AAV-2 rep 
      inhibited HIV LTR activity in both fibroblast and T-cell lines. The effect of 
      HHV-6 and AAV-2 rep genes on the HIV LTR was independent of the NF-kappa B, Sp1, 
      and TATA box elements. These results suggest that HHV-6 Rep is a multifunctional 
      regulatory protein with properties related to, but distinct from, those of AAV-2 
      Rep.
FAU - Thomson, B J
AU  - Thomson BJ
AD  - Department of Medicine, University of Cambridge, Addenbrooke's Hospital, UK.
FAU - Weindler, F W
AU  - Weindler FW
FAU - Gray, D
AU  - Gray D
FAU - Schwaab, V
AU  - Schwaab V
FAU - Heilbronn, R
AU  - Heilbronn R
LA  - eng
SI  - GENBANK/X83413
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 137750-19-7 (rep proteins, Adeno-associated virus 2)
SB  - IM
MH  - Cell Line
MH  - *DNA Replication
MH  - DNA, Viral/biosynthesis
MH  - DNA-Binding Proteins/*genetics/physiology
MH  - Dependovirus/*growth & development
MH  - Gene Expression Regulation, Viral/*genetics
MH  - Genes, Regulator/physiology
MH  - Genes, Viral/physiology
MH  - Genetic Complementation Test
MH  - HIV Long Terminal Repeat/genetics
MH  - Helper Viruses/*physiology
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Sequence Deletion/physiology
MH  - Sequence Homology, Nucleic Acid
MH  - Transcriptional Activation
MH  - Transfection
MH  - Viral Proteins/*genetics/physiology
MH  - Virus Replication/*genetics
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - S0042-6822(84)71535-2 [pii]
AID - 10.1006/viro.1994.1535 [doi]
PST - ppublish
SO  - Virology. 1994 Oct;204(1):304-11. doi: 10.1006/viro.1994.1535.