PMID- 8091672
OWN - NLM
STAT- MEDLINE
DCOM- 19941020
LR  - 20131121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 204
IP  - 1
DP  - 1994 Oct
TI  - The protein p30, encoded at the gag-pro junction of mouse mammary tumor virus, is 
      a dUTPase fused with a nucleocapsid protein.
PG  - 420-4
AB  - A ribosomal frameshift at the gag-pro junction of mouse mammary tumor virus 
      (MMTV) gives rise to the protein p30. The protein consists of two domains, the 
      zinc-finger-containing nucleocapsid (NC) protein portion with 95 residues and a 
      C-terminal extension comprising 154 residues. The C-terminal domain shows 
      similarity in sequence with the enzyme dUTPase from other sources. In this paper, 
      we demonstrate that p30 is a functional dUTPase. Overproduction of the NC protein 
      in Escherichia coli, using the native frameshift sequence at the gag stop codon, 
      caused a detectable expression of dUTPase ascribed to a low frequency of 
      readthrough. By a 1-base insertion, eliminating the gag stop codon and fusing the 
      gag and pro reading frames, a plasmid, pET-3d-NCDU, directing overexpression of 
      p30, was constructed. The overproduced protein, purified by phosphocellulose 
      chromatography, shows both zinc-binding and dUTPase activity. Analytical gel 
      filtration and sequence homology to other dUTPases suggest a trimeric assembly of 
      p30 subunits. MMTV thus possesses two different forms of the nucleocapsid 
      protein, the ordinary NC protein and the p30, having the NC protein connected to 
      a domain of dUTPase.
FAU - Bergman, A C
AU  - Bergman AC
AD  - Department of Biochemistry, University of Lund, Sweden.
FAU - Bjornberg, O
AU  - Bjornberg O
FAU - Nord, J
AU  - Nord J
FAU - Nyman, P O
AU  - Nyman PO
FAU - Rosengren, A M
AU  - Rosengren AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Gene Products, gag)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Core Proteins)
RN  - EC 3.6.1.- (Pyrophosphatases)
RN  - EC 3.6.1.23 (dUTP pyrophosphatase)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Capsid/genetics/*metabolism
MH  - Frameshift Mutation/physiology
MH  - Gene Products, gag/*genetics
MH  - Mammary Tumor Virus, Mouse/*chemistry/enzymology/genetics
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Pyrophosphatases/genetics/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Viral Core Proteins/genetics/*metabolism
MH  - Zinc/metabolism
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - S0042-6822(84)71547-9 [pii]
AID - 10.1006/viro.1994.1547 [doi]
PST - ppublish
SO  - Virology. 1994 Oct;204(1):420-4. doi: 10.1006/viro.1994.1547.