PMID- 8093491
OWN - NLM
STAT- MEDLINE
DCOM- 19930211
LR  - 20211203
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 2
DP  - 1993 Feb
TI  - Isolation of a human T-lymphotropic virus type I strain from Australian 
      aboriginals.
PG  - 843-51
AB  - A human T-lymphotropic virus type I (HTLV-I) strain was isolated in a CD4+ 
      T-lymphocyte culture established from a healthy seropositive Australian 
      Aboriginal. This isolate, identified as HTLV-IMSHR-1, was detected by 
      immunofluorescence with monoclonal antibodies, by the presence of gag-encoded 
      protein p24 in the culture supernatant, and by cocultivation leading to infection 
      and transformation of lymphocytes from an HTLV-I-negative donor. By using the 
      polymerase chain reaction technique, the env gene and segments of the pol and pX 
      regions of the proviral genome of HTLV-I(MSHR-1) were amplified and sequenced. 
      Comparison with the envelope sequences of prototype strains revealed up to 7% 
      divergence at the nucleotide level and 3.1 to 4.3% divergence in the predicted 
      amino acid sequence. Phylogenetic analysis showed that the Australian and 
      Melanesian isolates are related. Differential reactivity with monoclonal 
      antibodies suggests that gag protein p19 of HTLV-I(MSHR-1) is also divergent. The 
      potential for antigenic divergence between the prototype HTLV-I isolates and the 
      Austro-Melanesian variants requires further investigation, because it would have 
      implications for serodiagnosis and vaccine development.
FAU - Bastian, I
AU  - Bastian I
AD  - Menzies School of Health Research, Casuarina, Darwin, Northern Territory, 
      Australia.
FAU - Gardner, J
AU  - Gardner J
FAU - Webb, D
AU  - Webb D
FAU - Gardner, I
AU  - Gardner I
LA  - eng
SI  - GENBANK/M92818
SI  - GENBANK/M92819
SI  - GENBANK/M92820
SI  - GENBANK/M92821
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Viral)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (internal protein p24, Human T-lymphotropic virus 2)
SB  - IM
GS  - env
GS  - gag
GS  - pol
MH  - Amino Acid Sequence
MH  - Antibodies, Viral/blood/pharmacology
MH  - Australia/epidemiology
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/microbiology
MH  - Cell Fusion/drug effects
MH  - Cells, Cultured
MH  - Fluorescent Antibody Technique
MH  - Genes, env/*genetics
MH  - Genes, pol/*genetics
MH  - Human T-lymphotropic virus 1/*isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Native Hawaiian or Other Pacific Islander
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Retroviridae Proteins, Oncogenic/isolation & purification
MH  - Sequence Homology, Amino Acid
PMC - PMC237438
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
PMCR- 1993/02/01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PHST- 1993/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.2.843-851.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Feb;67(2):843-51. doi: 10.1128/JVI.67.2.843-851.1993.