PMID- 8093625
OWN - NLM
STAT- MEDLINE
DCOM- 19930217
LR  - 20211203
IS  - 0022-3085 (Print)
IS  - 0022-3085 (Linking)
VI  - 78
IP  - 2
DP  - 1993 Feb
TI  - Human-specific c-neu proto-oncogene protein overexpression in human malignant 
      astrocytomas before and after xenografting.
PG  - 240-51
AB  - Overexpression of human-specific c-neu proto-oncogene transmembrane tyrosine 
      kinase receptor protein (p185) is an index of cell transformation and of poor 
      patient survival in several malignancies. The authors studied this protein in 
      low- and high-grade human malignant astrocytomas before and after xenografting 
      into aspiration pockets in rat cortex. Human-specific p185c-neu-positive cells 
      were found in tumor specimens from all grades of astrocytoma. Significantly fewer 
      p185c-neu-positive cells were observed in the low-grade versus the high-grade 
      astrocytomas examined (p < 0.05). Human specific p185c-neu-positive cells were 
      also positive for the human major histocompatibility complex, human leukocyte 
      antigen (HLA)-DR, as well as glial fibrillary acidic protein and S-100 protein. 
      Fresh suspensions of tumor tissue were prelabeled with the plant lectin Phaseolus 
      vulgaris leukoagglutinin and xenografted into pockets in rat cortex. A class of 
      human p185c-neu-positive cells found in tissue samples from all grades of 
      astrocytoma migrated in the rat brain along parallel and intersecting nerve fibre 
      bundles and basement membrane-lined surfaces. Migrated p185c-neu-positive cells 
      were also positive for HLA-DR, Phaseolus vulgaris leukoagglutinin, glial 
      fibrillary acidic protein, and S-100 protein, suggesting that they were in fact 
      human astrocytoma cells. Simultaneous expression of human p185c-neu, HLA-DR, and 
      glial fibrillary acidic protein was observed in a class of human malignant 
      astrocytoma cells in both tumor tissue and xenografted cells that migrated into 
      rat brain. These molecules are signature proteins for the study of the spread of 
      human malignant astrocytomas in an animal model, and indicate that transformed 
      human malignant astrocytoma cells can migrate within the parenchyma of the 
      central nervous system and could play a role in the development of multifocal 
      tumors.
FAU - Bernstein, J J
AU  - Bernstein JJ
AD  - Department of Neurological Surgery, George Washington University School of 
      Medicine, Washington, D.C.
FAU - Anagnostopoulos, A V
AU  - Anagnostopoulos AV
FAU - Hattwick, E A
AU  - Hattwick EA
FAU - Laws, E R Jr
AU  - Laws ER Jr
LA  - eng
GR  - CA48956/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosurg
JT  - Journal of neurosurgery
JID - 0253357
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Animals
MH  - Astrocytoma/*chemistry/pathology
MH  - Biomarkers, Tumor/analysis
MH  - Brain Neoplasms/*chemistry
MH  - Cell Transformation, Neoplastic/*chemistry
MH  - Glioblastoma/chemistry/pathology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Neoplasm Transplantation
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, ErbB-2
MH  - Transplantation, Heterologous
EDAT- 1993/02/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.3171/jns.1993.78.2.0240 [doi]
PST - ppublish
SO  - J Neurosurg. 1993 Feb;78(2):240-51. doi: 10.3171/jns.1993.78.2.0240.