PMID- 8093892
OWN - NLM
STAT- MEDLINE
DCOM- 19930301
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 177
IP  - 2
DP  - 1993 Feb 1
TI  - Superantigen-reactive CD4+ T cells are required to stimulate B cells after 
      infection with mouse mammary tumor virus.
PG  - 359-66
AB  - Superantigens are defined by their ability to stimulate a large fraction of T 
      cells via interaction with the T cell receptor (TCR) V beta domain. Endogenous 
      superantigens, classically termed minor lymphocyte-stimulating (Mls) antigens, 
      were recently identified as products of open reading frames (ORF) in integrated 
      proviral copies of mouse mammary tumor virus (MMTV). We have described an 
      infectious MMTV homologue of the classical endogenous superantigen Mls-1a 
      (Mtv-7). The ORF molecules of both the endogenous Mtv-7 and the infectious 
      MMTV(SW) interact with T cells expressing the TCR V beta 6, 7, 8.1, and 9 
      domains. Furthermore, the COOH termini of their ORF molecules, thought to confer 
      TCR specificity, are very similar. Since successful transport of MMTV from the 
      site of infection in the gut to the mammary gland depends on a functional immune 
      system, we were interested in determining the early events after and requirements 
      for MMTV infection. We show that MMTV(SW) infection induces a massive response of 
      V beta 6+ CDC4+ T cells, which interact with the viral ORF. Concomitantly, we 
      observed a B cell response and differentiation that depends on both the presence 
      and stimulation of the superantigen-reactive T cells. Furthermore, we show that B 
      cells are the main target of the initial MMTV infection as judged by the presence 
      of the reverse-transcribed viral genome and ORF transcripts. Thus, we suggest 
      that MMTV infection of B cells leads to ORF-mediated B-T cell interaction, which 
      maintains and possibly amplifies viral infection.
FAU - Held, W
AU  - Held W
AD  - Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
FAU - Shakhov, A N
AU  - Shakhov AN
FAU - Izui, S
AU  - Izui S
FAU - Waanders, G A
AU  - Waanders GA
FAU - Scarpellino, L
AU  - Scarpellino L
FAU - MacDonald, H R
AU  - MacDonald HR
FAU - Acha-Orbea, H
AU  - Acha-Orbea H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, Viral)
RN  - 0 (Minor Lymphocyte Stimulatory Antigens)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Antigens, Viral/immunology
MH  - B-Lymphocytes/*immunology/microbiology
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Gene Expression Regulation, Viral
MH  - Genes, Viral
MH  - *Lymphocyte Activation
MH  - Mammary Tumor Virus, Mouse/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Minor Lymphocyte Stimulatory Antigens/*immunology
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Tumor Virus Infections/*immunology/microbiology
MH  - Viral Structural Proteins/genetics
PMC - PMC2190911
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
PMCR- 1993/08/01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PHST- 1993/08/01 00:00 [pmc-release]
AID - 93147725 [pii]
AID - 10.1084/jem.177.2.359 [doi]
PST - ppublish
SO  - J Exp Med. 1993 Feb 1;177(2):359-66. doi: 10.1084/jem.177.2.359.