PMID- 8095009 OWN - NLM STAT- MEDLINE DCOM- 19930330 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 62 IP - 4 DP - 1993 TI - Physical localization of 70 polymorphic markers to human chromosome 5 by fluorescence in situ hybridization. PG - 207-10 AB - We used fluorescence in situ hybridization (FISH) to prepare a cytogenetic framework map of 21 polymorphic markers that had been used previously to construct a genetic linkage anchor map of chromosome 5. In addition, we localized 49 other markers that have been genotyped on CEPH families. This study demonstrates that FISH can be used to confirm genetic linkage data, and that it can provide a means of determining the cytogenetic locations and relative order of markers whose order could not be assigned by genetic linkage analysis alone. The cytogenetic map prepared by FISH may help to identify probes of interest for regional mapping studies. FAU - Neuman, W L AU - Neuman WL AD - Department of Medicine, University of Chicago, IL 60637. FAU - Westbrook, C A AU - Westbrook CA FAU - Dixon, M AU - Dixon M FAU - Espinosa, R 3rd AU - Espinosa R 3rd FAU - Patel, Y D AU - Patel YD FAU - Nakamura, Y AU - Nakamura Y FAU - Weiffenbach, B AU - Weiffenbach B FAU - Le Beau, M M AU - Le Beau MM LA - eng GR - CA40046/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (DNA Probes) RN - 0 (Genetic Markers) SB - IM MH - *Chromosome Mapping MH - *Chromosomes, Human, Pair 5 MH - Cosmids MH - DNA Probes MH - *Genetic Markers MH - Humans MH - In Situ Hybridization, Fluorescence MH - Polymorphism, Restriction Fragment Length EDAT- 1993/01/01 00:00 MHDA- 1993/01/01 00:01 CRDT- 1993/01/01 00:00 PHST- 1993/01/01 00:00 [pubmed] PHST- 1993/01/01 00:01 [medline] PHST- 1993/01/01 00:00 [entrez] AID - 10.1159/000133477 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1993;62(4):207-10. doi: 10.1159/000133477.