PMID- 8095337
OWN - NLM
STAT- MEDLINE
DCOM- 19930408
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 5
DP  - 1993 Mar 1
TI  - GD2 ganglioside on human T-lymphotropic virus type I-infected T cells: possible 
      activation of beta-1,4-N-acetylgalactosaminyltransferase gene by p40tax.
PG  - 1972-6
AB  - Ganglioside expression on adult T-cell leukemia (ATL) and human T-cell 
      lymphotropic virus type I (HTLV-I)-infected cells was determined by using a panel 
      of monoclonal antibodies. ATL lines and HTLV-I-infected cells specifically 
      expressed GD2. Leukemia cells from ATL patients generally expressed low levels of 
      GD2 but the percentage of GD2+ cells increased up to 40-70% after in vitro 
      culture in the presence of interleukin 2 for about a week. No other type of 
      leukemia cells and normal peripheral T cells expressed GD2 during in vitro 
      culture under the same conditions. The appearance of GD2 in the cultured ATL 
      cells corresponded with the expression of p40tax, a product of the HTLV-I gene. 
      Peripheral lymphocytes infected with a p40tax-expressing retroviral vector 
      expressed high levels of GD2 in comparison with control lymphocytes containing 
      the neomycin-resistance gene alone. The apparently increased levels of 
      beta-1,4-N-acetylgalactosaminyltransferase (GM2/GD2 synthase) mRNA in these cells 
      were demonstrated by reverse transcription-polymerase chain reaction analysis. 
      Concordance between mRNA expression for the HTLV-I tax1/rex1 genes and the 
      beta-1,4-N-acetylgalactosaminyltransferase gene was also observed in uncultured 
      ATL cells. These results suggest that high GD2 expression was due to neosynthesis 
      from precursor GD3 by increased expression of this enzyme induced by p40tax in 
      vitro and in vivo.
FAU - Furukawa, K
AU  - Furukawa K
AD  - Department of Oncology, Nagasaki University School of Medicine, Japan.
FAU - Akagi, T
AU  - Akagi T
FAU - Nagata, Y
AU  - Nagata Y
FAU - Yamada, Y
AU  - Yamada Y
FAU - Shimotohno, K
AU  - Shimotohno K
FAU - Cheung, N K
AU  - Cheung NK
FAU - Shiku, H
AU  - Shiku H
FAU - Furukawa, K
AU  - Furukawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Gangliosides)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 65988-71-8 (ganglioside, GD2)
RN  - EC 2.4.1.- (N-Acetylgalactosaminyltransferases)
SB  - IM
MH  - Base Sequence
MH  - Deltaretrovirus Infections/genetics/*metabolism
MH  - Enzyme Induction
MH  - Gangliosides/*metabolism
MH  - Gene Expression
MH  - Gene Expression Regulation, Leukemic
MH  - Genes, pX
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia, T-Cell/enzymology/genetics/microbiology
MH  - Molecular Sequence Data
MH  - N-Acetylgalactosaminyltransferases/*genetics
MH  - Oligodeoxyribonucleotides/chemistry
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - T-Lymphocytes/metabolism/*microbiology
MH  - Tumor Cells, Cultured
PMC - PMC46002
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
PMCR- 1993/09/01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
PHST- 1993/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.90.5.1972 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1972-6. doi: 10.1073/pnas.90.5.1972.