PMID- 8096222
OWN - NLM
STAT- MEDLINE
DCOM- 19930429
LR  - 20190825
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 43
IP  - 1-2
DP  - 1993 Mar
TI  - Cytokine-regulated adhesion between encephalitogenic T lymphocytes and 
      cerebrovascular endothelial cells.
PG  - 23-30
AB  - Adhesive interactions between murine cerebrovascular endothelial cells (EC) which 
      comprise the blood-brain barrier (BBB) and myelin basic protein (MBP)-specific 
      encephalitogenic T lymphocytes were investigated. Adhesion was assessed by 
      measuring the percent attachment of 51Cr-labeled T cells to EC monolayers. The 
      basal level adhesion (20-35%) was significantly up-regulated by treating EC with 
      recombinant murine gamma interferon (IFN-gamma), interleukin-1 alpha (IL-1 alpha) 
      and/or tumor necrosis factor-alpha (TNF alpha). The ability of these cytokines to 
      modulate adhesion was dose- and time-dependent and could be detected as early as 
      1 h after treatment. The expression of intercellular adhesion molecule-1 (ICAM-1) 
      by EC was examined by immunofluorescence staining and ELISA. Although all 
      unstimulated EC cultures expressed ICAM-1, treatment of EC with the above 
      cytokines dramatically up-regulated the level of ICAM-1 expression in a dose- and 
      time-dependent fashion similar to that observed in the adhesion assays. Treatment 
      of EC with transforming growth factor-beta 1 (TGF beta) down-regulated the level 
      of T cell adhesion on untreated EC in a dose-dependent manner. Pretreatment of EC 
      with TGF beta also partially inhibited the up-regulation of adhesion induced by 
      IFN-gamma, IL-1 alpha and/or TNF alpha. TGF beta had no effect on the 
      up-regulation of ICAM-1 expression induced by IFN-gamma, IL-1 alpha and/or TNF 
      alpha. These results indicate that in addition to ICAM-1, other molecules may be 
      involved in adhesion of encephalitogenic T cells to the EC comprising the 
      cerebral vasculature.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - McCarron, R M
AU  - McCarron RM
AD  - Stroke Branch, NINDS, National Institutes of Health, Bethesda, MD 20892.
FAU - Wang, L
AU  - Wang L
FAU - Racke, M K
AU  - Racke MK
FAU - McFarlin, D E
AU  - McFarlin DE
FAU - Spatz, M
AU  - Spatz M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Transforming Growth Factor beta)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Brain/*blood supply
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/analysis/*physiology
MH  - Cells, Cultured
MH  - Cytokines/*pharmacology
MH  - Encephalomyelitis, Autoimmune, Experimental/*etiology/immunology
MH  - Endothelium, Vascular/cytology/*physiology
MH  - Female
MH  - Intercellular Adhesion Molecule-1
MH  - Mice
MH  - T-Lymphocytes/*physiology
MH  - Transforming Growth Factor beta/pharmacology
EDAT- 1993/03/01 00:00
MHDA- 2000/06/01 09:00
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 0165-5728(93)90071-6 [pii]
AID - 10.1016/0165-5728(93)90071-6 [doi]
PST - ppublish
SO  - J Neuroimmunol. 1993 Mar;43(1-2):23-30. doi: 10.1016/0165-5728(93)90071-6.