PMID- 8097107
OWN - NLM
STAT- MEDLINE
DCOM- 19930520
LR  - 20191023
IS  - 0937-9827 (Print)
IS  - 0937-9827 (Linking)
VI  - 105
IP  - 5
DP  - 1993
TI  - Simultaneous identification of amphetamine and its derivatives in urine using 
      HPLC-UV.
PG  - 265-9
AB  - An HPLC-UV method for the simultaneous identification of amphetamine, 
      methamphetamine, 3,4-methylenedioxyamphetamine (MDA) and 
      3,4-methylenedioxymethamphetamine (MDMA) in urine is described. It includes a 
      rapid extraction procedure of the 4 analogs from urine using Extrelut 3 columns, 
      derivatization with sodium 1,2-naphthoquinone-4-sulphonate (NQS) to obtain highly 
      chromophoric UV-VIS derivatives, and a final HPLC analysis using an ion-pair 
      reversed-phase technique with eluent monitoring at 480 nm. Structural 
      characterization of the derivatives obtained by mass spectrometry is reported. 
      Recoveries of the amphetamines were in the range 80-85% at concentrations of 300 
      ng/ml. Practical detection limits were 40-60 ng/ml (S/N ratio = 10) for all 
      derivatives. The chromatographic peaks of the NQS derivatized amphetamines are 
      fairly narrow and well resolved. The method is simple, rapid, quite sensitive, 
      and specific for convenient confirmation of preliminary positive results obtained 
      with immunoassays.
FAU - Tedeschi, L
AU  - Tedeschi L
AD  - Centre of Behavioural and Forensic Toxicology, Istituto Medicina Legale, 
      University of Padova, Italy.
FAU - Frison, G
AU  - Frison G
FAU - Castagna, F
AU  - Castagna F
FAU - Giorgetti, R
AU  - Giorgetti R
FAU - Ferrara, S D
AU  - Ferrara SD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - CK833KGX7E (Amphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analogs & derivatives/*analysis
MH  - Amphetamine/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Methamphetamine/*analysis
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Substance Abuse Treatment Centers
MH  - Substance-Related Disorders/diagnosis/*urine
MH  - Ultraviolet Rays
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF01370383 [doi]
PST - ppublish
SO  - Int J Legal Med. 1993;105(5):265-9. doi: 10.1007/BF01370383.