PMID- 8097889
OWN - NLM
STAT- MEDLINE
DCOM- 19930603
LR  - 20190830
IS  - 0167-0115 (Print)
IS  - 0167-0115 (Linking)
VI  - 44
IP  - 1
DP  - 1993 Mar 5
TI  - The effects of transforming growth factor alpha and somatostatin on regenerating 
      hepatocytes in the rat.
PG  - 49-59
AB  - Transforming growth factor alpha (TGF alpha) stimulates DNA synthesis in adult 
      rat hepatocytes, and plays a physiological role after partial hepatectomy by an 
      autocrine mechanism. Somatostatin (SS-14) is a potent inhibitor of 
      gastrointestinal function and inhibits proliferation in various cell types. We 
      examined the proliferative effect of TGF alpha and the inhibitory effect of SS-14 
      on hepatocytes isolated at various times after partial hepatectomy. To study the 
      mechanism of SS-14 further, we treated rats with the long acting SS-14 analog, 
      octreotide, before or after 70% hepatectomy to determine whether or not a 
      differential effect could be seen. We confirmed the proliferative effects of TGF 
      alpha, and the inhibitory action of SS-14 in the early phase of liver 
      regeneration in vitro. Regenerating hepatocytes isolated from hepatectomized 
      livers respond to TGF alpha only at early time points (2 h) but do not respond to 
      SS-14. In addition, the long acting SS-14 analog, octreotide, inhibited hepatic 
      regeneration only when administered prior to hepatectomy. We conclude that 
      exogenous peptide stimulation is effective only in the early phase of the hepatic 
      proliferative response. After the initial changes brought about by hepatectomy, 
      subsequent steps of the regenerative process appear refractory to external 
      stimuli.
FAU - Hashimoto, M
AU  - Hashimoto M
AD  - Department of Surgery, University of Michigan Medical School, Ann Arbor.
FAU - Kothary, P C
AU  - Kothary PC
FAU - Raper, S E
AU  - Raper SE
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Regul Pept
JT  - Regulatory peptides
JID - 8100479
RN  - 0 (Growth Inhibitors)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 51110-01-1 (Somatostatin)
RN  - 9007-49-2 (DNA)
RN  - RWM8CCW8GP (Octreotide)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA/biosynthesis/drug effects
MH  - Growth Inhibitors/pharmacology
MH  - Hepatectomy
MH  - Liver/cytology/drug effects/metabolism
MH  - Liver Regeneration/*drug effects
MH  - Male
MH  - Octreotide/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Somatostatin/*pharmacology
MH  - Transforming Growth Factor alpha/*pharmacology
EDAT- 1993/03/05 00:00
MHDA- 1993/03/05 00:01
CRDT- 1993/03/05 00:00
PHST- 1993/03/05 00:00 [pubmed]
PHST- 1993/03/05 00:01 [medline]
PHST- 1993/03/05 00:00 [entrez]
AID - 0167-0115(93)90129-V [pii]
AID - 10.1016/0167-0115(93)90129-v [doi]
PST - ppublish
SO  - Regul Pept. 1993 Mar 5;44(1):49-59. doi: 10.1016/0167-0115(93)90129-v.