PMID- 8100196
OWN - NLM
STAT- MEDLINE
DCOM- 19930729
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 235
IP  - 1
DP  - 1993 Apr 22
TI  - Integrative transporter-mediated release from cytoplasmic and vesicular 
      5-hydroxytryptamine stores in cultured neurons.
PG  - 51-7
AB  - The direct effects of 3,4-methylenedioxymethamphetamine (MDMA) and 
      p-chloroamphetamine (PCA) were studied in microculture of fetal 
      5-hydroxytryptamine (5-HT) neurons. Both MDMA and PCA released 5-HT with the 
      potency of PCA > MDMA by a mechanism inhibited by fluoxetine, and inhibitor of 
      the 5-HT transporter. The transporter-mediated release by MDMA and PCA reduced 
      intracellular stores of 5-HT. Both MDMA and PCA inhibit MAO-A activities, which 
      also contributes to the increase of extracellular 5-HT levels. Deprenyl (10(-7) 
      M) increased the amount of intracellular 5-HT and potentiated the MDMA- or 
      PCA-induced release of 5-HT. Conversely, reserpine (10(-9) M) reduced the 
      intracellular 5-HT levels and attenuated the transporter-mediated release. In 
      addition, MDMA- or PCA-mediated release was attenuated by nimodipine (10(-8) M), 
      an L-type Ca2+ channel antagonist. Our results indicate that MDMA- or PCA-induced 
      release of 5-HT occurs from the cytoplasm to the media through the 5-HT 
      transporter, and that the release may incorporate 5-HT from the vesicular stores.
FAU - Gu, X F
AU  - Gu XF
AD  - Department of Biology, New York University, NY 10003.
FAU - Azmitia, E C
AU  - Azmitia EC
LA  - eng
GR  - NIDA 271-90-7403/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Slc6a4 protein, rat)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 2K1V7GP655 (Selegiline)
RN  - 333DO1RDJY (Serotonin)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 57WA9QZ5WH (Nimodipine)
RN  - 64-12-0 (p-Chloroamphetamine)
RN  - 8B1QWR724A (Reserpine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - LYJ16FZU9Q (Clorgyline)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Brain Stem/cytology/drug effects
MH  - Carrier Proteins/*metabolism
MH  - Cells, Cultured
MH  - Clorgyline/pharmacology
MH  - Female
MH  - Fluoxetine/pharmacology
MH  - Membrane Glycoproteins/*metabolism
MH  - *Membrane Transport Proteins
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - *Nerve Tissue Proteins
MH  - Neurons/*drug effects/metabolism
MH  - Nimodipine/pharmacology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reserpine/pharmacology
MH  - Selegiline/pharmacology
MH  - Serotonin/*metabolism
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - p-Chloroamphetamine/*pharmacology
EDAT- 1993/04/22 00:00
MHDA- 1993/04/22 00:01
CRDT- 1993/04/22 00:00
PHST- 1993/04/22 00:00 [pubmed]
PHST- 1993/04/22 00:01 [medline]
PHST- 1993/04/22 00:00 [entrez]
AID - 0014-2999(93)90819-4 [pii]
AID - 10.1016/0014-2999(93)90819-4 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1993 Apr 22;235(1):51-7. doi: 10.1016/0014-2999(93)90819-4.