PMID- 8100641
OWN - NLM
STAT- MEDLINE
DCOM- 19930805
LR  - 20190814
IS  - 0036-5521 (Print)
IS  - 0036-5521 (Linking)
VI  - 28
IP  - 6
DP  - 1993 Jun
TI  - Effect of 5-aminosalicylic acid and analogous substances on superoxide generation 
      and intracellular free calcium in human neutrophilic granulocytes.
PG  - 527-32
AB  - Activated polymorphonuclear leukocytes (PMNs), which are found in the 
      inflammatory lesions of chronic inflammatory bowel disease, produce 
      tissue-destructive oxygen-derived free radicals. The influence of 
      5-aminosalicylic acid (5-ASA), its acetylated metabolite (Ac-5-ASA), 
      sulfasalazine (SAZ), and olsalazine (OLZ) (5-ASA dimer linked by an azo group) in 
      pharmacologically relevant concentrations (0.1-10 mM) were tested on PMN 
      superoxide production with either the receptor-specific agent 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or the protein kinase C activator 
      phorbol myristate acetate (PMA). Inhibition of receptor-specific superoxide 
      production occurred at 0.07, 0.32, and 0.63 mM (IC50 values) for 5-ASA, SAZ, and 
      OLZ, respectively. No inhibitory effects of SAZ and OLZ were observed when PMA 
      was applied as stimulus for PMN superoxide production. The results indicate that 
      the signal to which PMNs respond by generating superoxide is primarily due to 
      calcium release from intracellular stores. They further suggest that SAZ and OLZ 
      may affect the oxygen-derived free radical production in human PMNs by unspecific 
      cytotoxicity or by interference with the nicotinamide adenine dinucleotide 
      phosphate, reduced (NADPH) oxidase system, whereas 5-ASA itself is a free radical 
      scavenger.
FAU - Nielsen, O H
AU  - Nielsen OH
AD  - Dept. of Medical Gastroenterology C, Herlev Hospital, University of Copenhagen, 
      Denmark.
FAU - Bouchelouche, P N
AU  - Bouchelouche PN
FAU - Berild, D
AU  - Berild D
FAU - Ahnfelt-Ronne, I
AU  - Ahnfelt-Ronne I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Aminosalicylic Acids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Free Radicals)
RN  - 11062-77-4 (Superoxides)
RN  - 3XC8GUZ6CB (Sulfasalazine)
RN  - 4Q81I59GXC (Mesalamine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9H126Z3PF5 (N-acetyl-5-aminosalicylic acid)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
RN  - ULS5I8J03O (olsalazine)
RN  - Y5V2N1KE8U (Sulfapyridine)
SB  - IM
MH  - Aminosalicylic Acids/*pharmacology
MH  - Calcium/*metabolism
MH  - Free Radical Scavengers
MH  - Free Radicals
MH  - Humans
MH  - In Vitro Techniques
MH  - Mesalamine
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/metabolism
MH  - Sulfapyridine/pharmacology
MH  - Sulfasalazine/pharmacology
MH  - Superoxides/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.3109/00365529309098261 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 1993 Jun;28(6):527-32. doi: 10.3109/00365529309098261.