PMID- 8103091
OWN - NLM
STAT- MEDLINE
DCOM- 19930929
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 70
IP  - 1
DP  - 1993 Jul
TI  - The excitatory and inhibitory amino acid receptors on horizontal cells isolated 
      from the white perch retina.
PG  - 8-19
AB  - 1. The distribution and the properties of receptors to the inhibitory amino acid 
      glycine (GLY) and the excitatory amino acid glutamate (GLU) and its analogues 
      kainate (KA), quisqualate (QUIS), 
      alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and 
      N-methyl-D-aspartate (NMDA), were studied with whole-cell and outside-out 
      patch-clamp techniques on all four types of horizontal cells isolated from the 
      retina of white perch. 2. Glycine at concentrations above 30 microM evoked 
      whole-cell current responses from two types of horizontal cells (H2 and H4). The 
      other two types of horizontal cells (H1 and H3) were unresponsive to GLY (30 
      microM-3 mM). 3. Responses elicited by GLY from H2 and H4 cells were similar, 
      consisting of inward currents that desensitized with a half-decay time of 0.5-2 s 
      at glycine concentrations between 100 and 500 microM. GLY-activated currents were 
      inhibited by the glycine receptor antagonist strychnine (STRYCH). Current 
      responses evoked by GLY reversed at the Cl- equilibrium potential. 4. 
      Dose-response analysis of peak currents induced by GLY revealed a Hill 
      coefficient of 2.0 +/- 0.1 (mean +/- SD, n = 3) and an median effective 
      concentration (EC50) of 85 +/- 2 microM (n = 3). 5. Single glycine receptor 
      channels recorded from outside-out patches had a main-state conductance of 47 +/- 
      4 pS (n = 3). 6. Every type of horizontal cell from the white perch responded to 
      GLU, KA, QUIS, and AMPA but none responded to exogenously applied NMDA (200 
      microM) or NMDA (200 microM) + GLY (1 microM) in a Mg+2-free bathing solution. 7. 
      The ratio of the amplitude of responses to GLU, KA, QUIS, and AMPA remained 
      nearly constant among all the horizontal cells tested, suggesting there might be 
      only a single population of non-NMDA receptors on these cells. 8. QUIS and KA 
      both elicited responses from the horizontal cells. When applied together with KA, 
      QUIS competitively antagonized the responses of horizontal cells to KA. 9. The 
      results demonstrated the existence of an inhomogeneous distribution of 
      strychnine-sensitive glycine receptors and a homogeneous distribution of non-NMDA 
      type glutamate receptors among the four types of white perch horizontal cells.
FAU - Zhou, Z J
AU  - Zhou ZJ
AD  - Jules Stein Eye Institute, UCLA School of Medicine 90024.
FAU - Fain, G L
AU  - Fain GL
FAU - Dowling, J E
AU  - Dowling JE
LA  - eng
GR  - EY-00824/EY/NEI NIH HHS/United States
GR  - EY-01844/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Amino Acids)
RN  - 0 (Glutamates)
RN  - 0 (Receptors, Amino Acid)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, Glycine)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - H9Y79VD43J (Strychnine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Amino Acids/pharmacology/*physiology
MH  - Animals
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Glutamates/pharmacology/physiology
MH  - Glutamic Acid
MH  - Glycine/pharmacology/physiology
MH  - Membrane Potentials/drug effects/physiology
MH  - Neural Inhibition/drug effects/*physiology
MH  - Perches
MH  - Receptors, Amino Acid/drug effects/*physiology
MH  - Receptors, Glutamate/drug effects/physiology
MH  - Receptors, Glycine
MH  - Receptors, N-Methyl-D-Aspartate/drug effects/physiology
MH  - Receptors, Neurotransmitter/drug effects/physiology
MH  - Retina/*cytology
MH  - Species Specificity
MH  - Strychnine/pharmacology
MH  - Synaptic Transmission/drug effects/*physiology
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1152/jn.1993.70.1.8 [doi]
PST - ppublish
SO  - J Neurophysiol. 1993 Jul;70(1):8-19. doi: 10.1152/jn.1993.70.1.8.