PMID- 8104826
OWN - NLM
STAT- MEDLINE
DCOM- 19931117
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 332
IP  - 1-2
DP  - 1993 Oct 11
TI  - The 5' untranslated region of the human gamma-glutamyl transferase mRNA contains 
      a tissue-specific active translational enhancer.
PG  - 88-92
AB  - We report the functional and structural analysis of the 5' untranslated region 
      (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient 
      expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell 
      lines shows that this 5'UTR acts as a tissue-specific translational enhancer. 
      Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by 
      RNase protection. Computer analysis of this 5'UTR detected the existence of a 
      stable stem and loop structure containing multiple steroid modulatory elements.
FAU - Diederich, M
AU  - Diederich M
AD  - Centre du Medicament, URA CNRS 597, Nancy, France.
FAU - Wellman, M
AU  - Wellman M
FAU - Visvikis, A
AU  - Visvikis A
FAU - Puga, A
AU  - Puga A
FAU - Siest, G
AU  - Siest G
LA  - eng
GR  - NIEHS ES06096/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (RNA, Messenger)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *Enhancer Elements, Genetic
MH  - Humans
MH  - Luciferases/genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/chemistry/*genetics
MH  - Tumor Cells, Cultured
MH  - beta-Galactosidase/genetics
MH  - gamma-Glutamyltransferase/*genetics
EDAT- 1993/10/11 00:00
MHDA- 1993/10/11 00:01
CRDT- 1993/10/11 00:00
PHST- 1993/10/11 00:00 [pubmed]
PHST- 1993/10/11 00:01 [medline]
PHST- 1993/10/11 00:00 [entrez]
AID - 0014-5793(93)80490-L [pii]
AID - 10.1016/0014-5793(93)80490-l [doi]
PST - ppublish
SO  - FEBS Lett. 1993 Oct 11;332(1-2):88-92. doi: 10.1016/0014-5793(93)80490-l.