PMID- 8105609
OWN - NLM
STAT- MEDLINE
DCOM- 19931028
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 29
IP  - 1
DP  - 1993 Jul
TI  - Characterization of human T-lymphotropic virus type I- and II-infected T-cell 
      lines: antigenic, phenotypic, and genotypic analysis.
PG  - 59-70
AB  - Eighteen long-term T-cell lines were established from peripheral blood 
      mononuclear cells of individuals infected with human T-lymphotropic virus type I 
      (HTLV-I) or II (HTLV-II). These cell lines (10 HTLV-I and 8 HTLV-II), 
      representing diverse pathologic profiles and geographic regions, have been in 
      culture for over 6 months and have constitutively produced p24gag antigen. 
      Antigenic characterization of the cell lines by Western blot analysis 
      demonstrated that all but one produced gag (p24) and env (gp46 or gp52) 
      structural proteins; one HTLV-I-infected cell line exhibited an aberrant protein 
      profile. Phenotypic analysis of the HTLV-infected cell lines demonstrated 
      phenotypes consistent with activated T-cells (CD5+, CD25+, HLA-DR+). The 
      HTLV-I-infected cell lines were predominantly CD4+ (IR, FS, A212, SP, 1657, 1742, 
      3669, 1996, and 3614), whereas EG was CD8+. The HTLV-II-infected cell lines were 
      either CD4+ (H2A, Y17, G12.1), CD8+ (H1H, H2E, Y03, Y06), or both (H1B). 
      Restriction map analysis and subtyping of the viral genomes demonstrated 
      heterogeneity among these isolates. Of the HTLV-I-infected cell lines, six were 
      subtype II, one was subtype III and, on the basis of additional restriction 
      sites, another subtype, tentatively classified as subtype IV, could be identified 
      for three of the HTLV-I-infected cell lines. Of the HTLV-II-infected cell lines, 
      six were subtype HTLV-IIa and two were subtype HTLV-IIb. While the majority of 
      the cell lines resemble the prototypic HTLV-I-infected (MT-2) and 
      HTLV-II-infected (MoT) cell lines, the antigenic, phenotypic, and genotypic data 
      collectively demonstrate heterogeneity among viral isolates representing diverse 
      geographic regions.
FAU - Dezzutti, C S
AU  - Dezzutti CS
AD  - Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, 
      GA 30333.
FAU - Rudolph, D L
AU  - Rudolph DL
FAU - Roberts, C R
AU  - Roberts CR
FAU - Lal, R B
AU  - Lal RB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Deltaretrovirus Antigens)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Cell Line/*microbiology
MH  - Child
MH  - Deltaretrovirus Antigens/analysis
MH  - Demography
MH  - Female
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Genotype
MH  - Human T-lymphotropic virus 1/genetics/*growth & development/immunology
MH  - Human T-lymphotropic virus 2/genetics/*growth & development/immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Polymorphism, Restriction Fragment Length
MH  - Restriction Mapping
MH  - T-Lymphocytes/*microbiology
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 0168-1702(93)90125-7 [pii]
AID - 10.1016/0168-1702(93)90125-7 [doi]
PST - ppublish
SO  - Virus Res. 1993 Jul;29(1):59-70. doi: 10.1016/0168-1702(93)90125-7.