PMID- 8111120
OWN - NLM
STAT- MEDLINE
DCOM- 19940331
LR  - 20190904
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 4
IP  - 12
DP  - 1993 Dec
TI  - Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine 
      chromosomes.
PG  - 720-7
AB  - To identify physical and genetic anchor loci on bovine chromosomes, 13 cosmids, 
      obtained after the screening of partial bovine cosmid libraries with the (CA)n 
      microsatellite motif, were mapped by fluorescence in situ hybridization (FISH). 
      Eleven cosmid probes yielded a specific signal on one of the bovine chromosomes 
      and identified the following loci: D5S2, D5S3, D6S3, D8S1, D11S5, D13S1, D16S5, 
      D17S2, D19S2, D19S3, D21S8. Two cosmids produced centromeric signals on many 
      chromosomes. The microsatellite-containing regions were subcloned and sequenced. 
      The sequence information revealed that the two centromeric cosmids were derived 
      from bovine satellites 1.723 and 1.709, respectively. A cosmid located in the 
      subtelomeric region of Chromosome (Chr) 17 (D17S2) had features of a 
      chromosome-specific satellite. Primers were designed for eight of the 
      nonsatellite cosmids, and seven of these microsatellites were polymorphic with 
      between three and eight alleles on a set of outbred reference families. The 
      polymorphic and chromosomally mapped loci can now be used to physically anchor 
      other bovine polymorphic markers by linkage analysis. The microsatellite primers 
      were also applied to DNA samples of a previously characterized panel of somatic 
      hybrid cell lines, allowing the assignment of seven microsatellite loci to 
      defined syntenic groups. These assignments confirmed earlier mapping results, 
      revealed a probable case of false synteny, and placed two formerly unassigned 
      syntenic groups on specific chromosomes.
FAU - Toldo, S S
AU  - Toldo SS
AD  - Department of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum, 
      Zurich.
FAU - Fries, R
AU  - Fries R
FAU - Steffen, P
AU  - Steffen P
FAU - Neibergs, H L
AU  - Neibergs HL
FAU - Barendse, W
AU  - Barendse W
FAU - Womack, J E
AU  - Womack JE
FAU - Hetzel, D J
AU  - Hetzel DJ
FAU - Stranzinger, G
AU  - Stranzinger G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - Chromosome Mapping/*veterinary
MH  - Cloning, Molecular
MH  - *Cosmids
MH  - DNA, Satellite/*genetics
MH  - *Genetic Markers
MH  - Hybrid Cells
MH  - In Situ Hybridization/veterinary
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1007/BF00357796 [doi]
PST - ppublish
SO  - Mamm Genome. 1993 Dec;4(12):720-7. doi: 10.1007/BF00357796.