PMID- 8111734
OWN - NLM
STAT- MEDLINE
DCOM- 19940330
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 72
IP  - 1
DP  - 1994 Jan
TI  - Trisomy 8 detection in Ph+ CML patients using conventional cytogenetic and 
      interphase fluorescence in situ hybridization techniques.
PG  - 24-7
AB  - We examined bone marrow (BM) cells from 6 Philadelphia chromosome positive (Ph+) 
      chronic myeloid leukemia (CML) patients in advanced phase of the disease using 
      conventional cytogenetic techniques and fluorescence in situ hybridization (FISH) 
      for detection of an extra chromosome 8. All patients showed mosaicism for trisomy 
      8 as a secondary chromosome abnormality. For FISH, we used the D8Z5 probe 
      specific for the centromeric region of chromosome 8 and analyzed 300 interphase 
      nuclei and a variable number of mitoses for each patient. The percentages of 
      metaphases carrying trisomy 8 were similar with both techniques, whereas the 
      percentage of interphase nuclei showing three hybridization spots indicative of 
      trisomy 8 was significantly lower than that in metaphases. This finding suggests 
      that cells with a supernumerary chromosome 8 may have a cell cycle time shorter 
      than that of disomic cells.
FAU - Fugazza, G
AU  - Fugazza G
FAU - Bruzzone, R
AU  - Bruzzone R
FAU - Dejana, A M
AU  - Dejana AM
FAU - Patrone, F
AU  - Patrone F
FAU - Sessarego, M
AU  - Sessarego M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Blast Crisis
MH  - Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 8
MH  - Chromosomes, Human, Pair 9
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Middle Aged
MH  - Translocation, Genetic
MH  - *Trisomy
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 0165-4608(94)90104-X [pii]
AID - 10.1016/0165-4608(94)90104-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Jan;72(1):24-7. doi: 10.1016/0165-4608(94)90104-x.