PMID- 8113428
OWN - NLM
STAT- MEDLINE
DCOM- 19940328
LR  - 20191023
IS  - 1010-5182 (Print)
IS  - 1010-5182 (Linking)
VI  - 21
IP  - 8
DP  - 1993 Dec
TI  - DNA heterogeneity in metastasizing squamous cell head and neck cancer.
PG  - 348-50
AB  - This study was carried out to determine whether analysis of DNA content of tumour 
      cells (expressed as DNA-index: DI) from patients with head and neck squamous cell 
      cancer (HNSCC) and lung squamous cell cancer (LSCC) could be helpful in 
      distinguishing HNSCC patients with LSCC as a second primary from those in which 
      LSCC represents a distant metastasis. Based on the assumption that metastasizing 
      tumours retain their original DNA content, the same DI at both locations would 
      suggest LSCC to be a distant metastasis from HNSCC, whereas a difference in DI at 
      both tumour locations makes LSCC to be a second primary more likely. The study 
      comprised 21 cases with HNSCC as well as LSCC. However, the basic assumption that 
      the identity of a tumour can be inferred from its DI proved to be false as 7 of 
      the 21 cases were characterized by more than one DI signifying several tumour 
      cell populations. This DNA heterogeneity was further substantiated by differences 
      in DI between the primary tumour and cervical lymph node metastasis in a second 
      series composed of 16 HNSCC patients. These data indicate that due to variation 
      in DI within one and the same tumour, DNA-analysis does not offer reliable 
      information when trying to differentiate between lung cancer as a second primary 
      or a distant metastasis in HNSCC patients.
FAU - Slootweg, P J
AU  - Slootweg PJ
AD  - Department of Pathology, University Hospital, Utrecht, The Netherlands.
FAU - Giessen, M C
AU  - Giessen MC
FAU - Rutgers, D H
AU  - Rutgers DH
FAU - Wils, I S
AU  - Wils IS
LA  - eng
PT  - Journal Article
PL  - Scotland
TA  - J Craniomaxillofac Surg
JT  - Journal of cranio-maxillo-facial surgery : official publication of the European 
      Association for Cranio-Maxillo-Facial Surgery
JID - 8704309
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Squamous Cell/genetics/*secondary
MH  - DNA, Neoplasm/*analysis/genetics
MH  - Flow Cytometry
MH  - Head and Neck Neoplasms/genetics/*pathology
MH  - Humans
MH  - Lymphatic Metastasis/genetics
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - S1010-5182(05)80496-2 [pii]
AID - 10.1016/s1010-5182(05)80496-2 [doi]
PST - ppublish
SO  - J Craniomaxillofac Surg. 1993 Dec;21(8):348-50. doi: 
      10.1016/s1010-5182(05)80496-2.