PMID- 8114618
OWN - NLM
STAT- MEDLINE
DCOM- 19940325
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 20
IP  - 4
DP  - 1993 Dec
TI  - A2a adenosine receptor gene expression in developing rat brain.
PG  - 313-27
AB  - Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound 
      receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 
      adenosine receptor subtypes have been cloned recently. The present study 
      documents the developmental appearance of A2a adenosine receptor gene expression 
      in the rat brain. In situ hybridization using 35S-labeled RNA probes generated 
      from the rat A2a adenosine receptor cDNA revealed receptor gene expression in the 
      striatum on gestational day (GD) 14. Developmental alterations in the pattern of 
      receptor gene expression within the striatum suggest that this receptor mRNA is 
      expressed by striatal neurons soon after they complete neurogenesis. Transient 
      expression of the A2a adenosine receptor mRNA was observed in cerebral cortex, 
      subiculum, parafascicularis nucleus of the thalamus, facial nucleus, trigeminal 
      nucleus, locus coeruleus, area postrema, anterior pituitary gland and in the 
      fetal cerebral vasculature. The ganglia of cranial nerves V, VII, VIII, IX and X 
      expressed A2a adenosine receptor mRNA in fetuses; adults have not been examined. 
      A2a adenosine receptor mRNA was expressed in the carotid body and intermediate 
      lobe of the pituitary during development and also in adult rats. Northern blot 
      analysis revealed that the A2a adenosine receptor transcript is consistent in 
      size (ca 2.5 kb) across the developmental period examined (GD 14 through adult). 
      Previous studies in adult rats have reported that A2a adenosine receptor gene 
      expression is limited to a population of striatal medium spiny neurons. This 
      study documents early developmental expression of the A2a adenosine receptor gene 
      in the striatum and its transient expression elsewhere in the brain and cerebral 
      vasculature. If the A2a adenosine receptor mRNA is translated into receptor 
      protein shortly after the mRNA is expressed, adenosine could influence neuronal 
      differentiation, migration, synaptogenesis, and angiogenesis. Expression of A2a 
      adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe 
      of the pituitary gland similarly suggests novel sites of adenosine action during 
      development and in the adult.
FAU - Weaver, D R
AU  - Weaver DR
AD  - Laboratory of Developmental Chronobiology, Massachusetts General Hospital, Boston 
      02114.
LA  - eng
GR  - DK 42125/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Purinergic P1)
SB  - IM
MH  - Animals
MH  - Blood Vessels/chemistry
MH  - Brain/embryology/growth & development
MH  - *Brain Chemistry
MH  - Cranial Nerves/chemistry
MH  - Enamel Organ/chemistry
MH  - Ganglia/chemistry
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Meninges/chemistry
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Organ Specificity
MH  - Pituitary Gland/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P1/*biosynthesis/classification/genetics
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 0169-328X(93)90058-W [pii]
AID - 10.1016/0169-328x(93)90058-w [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1993 Dec;20(4):313-27. doi: 
      10.1016/0169-328x(93)90058-w.